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Investigation of MTHFR C677T Gene Polymorphism, Biochemical and Clinical Parameters in Turkish Migraine Patients: Association with Allodynia and Fatigue

Abstract

We investigated whether there is any relationship between biochemical and clinical parameters of migraine and methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphism, associated with the migraine subtypes, symptoms, and gender. A total of 150 migraine patients with and without aura (MA and MO) and 107 non-sufferers were included in the study. Biochemical and clinical parameters were measured and genetic analysis was performed. The MTFHR C677T genotype was significantly higher in the migraine group (p = 0.000). The CT genotype frequency of individuals with a family history of migraine was significantly higher (p = 0.025). This genotype frequency was higher in patients who suffer from compression, allodynia, fatigue, and sleeplessness (p = 0.027, 0.023, 0.006, and 0.05, respectively). Homocysteine and total cholesterol levels were significantly higher in the migraine group than the control group (p = 0.007 and 0.010, respectively). However, the other biochemical and clinical parameters did not differ from each other (p > 0.05), with only attack frequency being significantly higher in the MO group (p = 0.005). While the folate and HDL levels were significantly higher in females (p = 0.001 and 0.000, respectively), the homocysteine and triglyceride levels were significantly higher in males (p = 0.000 for each one). BMIs were significantly lower in the control than the migraine group (p = 0.021); however, an association between the C677T variant and BMI was not found (p = 0.787) in the migraine group. An association between the MTHFR C667T polymorphism and migraine susceptibility was found. Additional studies including genetic, clinic, and biochemical parameters should be conducted to better understand the disease.

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Acknowledgments

We especially wish to thank the migraine patients and healthy individuals from Duzce province of Turkey for their devoted participation in this study. This study was supported by a research project from the University of Duzce, Scientific Research Projects Department (Project Number: 2012.04.01.083 to Dr A. Bahadir), Duzce, Turkey. We also thank Dr. Handan Ankarali for providing statistical consultation, and Dr. M. Samil Kok and Mrs Sara Ann Wigglesworth for useful comments and suggestions on the language of this article.

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Authors declare that there is no conflict of interest.

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Correspondence to Anzel Bahadir.

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Bahadir, A., Eroz, R. & Dikici, S. Investigation of MTHFR C677T Gene Polymorphism, Biochemical and Clinical Parameters in Turkish Migraine Patients: Association with Allodynia and Fatigue. Cell Mol Neurobiol 33, 1055–1063 (2013). https://doi.org/10.1007/s10571-013-9972-1

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Keywords

  • Migraine
  • MTHFR C677T polymorphism
  • Biochemical and clinical parameters