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Olfactory Ensheathing Cell-Conditioned Medium Protects Astrocytes Exposed to Hydrogen Peroxide Stress

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Abstract

The purpose of this study was to observe the effects of olfactory ensheathing cell conditioned medium (OECCM) on damaged astrocytes after exposure to H2O2 in vitro. OECCM was used to treat astrocytes after injury, which was induced by exposure to 500 μmol/L H2O2 for 20 min. The cell morphology was then observed under a light microscope, cell viability assessed by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, cell ultrastructure observed with transmission electron microscopy (TEM), and apoptosis assessed by Annexin V staining followed by cytometry and Western blot. H2O2 induced severe damage to astrocytes as evidenced by decreased cell number, pathological changes in cell morphology, and significantly elevated cell apoptosis. Cells incubated with OECCM displayed significantly improved cell viability and decreased cell apoptotic rate. Under TEM, H2O2-treated cells showed partially broken plasma membranes, swollen rough endoplasmic reticula, visible vacuoles, and swollen or deformed mitochondria with ruptured cristae. Incubation with OECCM significantly ameliorated these pathological changes in astrocytes. These results suggest that OECCM may protect astrocytes from oxidative damage by promoting cell survival while reducing apoptosis of the damaged cells.

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Acknowledgments

This study was supported by the grants from Jiangsu Province Health Department (KF200956).

Conflict of interest

The authors declared that they have no conflict of interest.

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Authors and Affiliations

  1. Department of Orthopedics, The Third Affiliated Hospital of Suzhou University, Changzhou, 213003, Jiangsu, China

    Liu Jinbo, Liu Zhiyuan & Ding WenGe

  2. School of Medicine, Jiangsu University, Zhenjiang, 212013, China

    Zhang Zhijian

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  1. Liu Jinbo
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  2. Liu Zhiyuan
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  3. Zhang Zhijian
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  4. Ding WenGe
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Correspondence to Ding WenGe.

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Jinbo, L., Zhiyuan, L., Zhijian, Z. et al. Olfactory Ensheathing Cell-Conditioned Medium Protects Astrocytes Exposed to Hydrogen Peroxide Stress. Cell Mol Neurobiol 33, 699–705 (2013). https://doi.org/10.1007/s10571-013-9937-4

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  • DOI: https://doi.org/10.1007/s10571-013-9937-4

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