Abstract
The presence of protein aggregates is common in neurodegenerative disorders; however, the real cause and effect of these aggregates during neurodegeneration is still a matter of investigation. We hypothesize that impairment of intracellular traffic may appear in the absence of protein inclusions and might trigger protein aggregation. In the present study, we aimed to evaluate mitochondria mobility as well as protein and messenger RNA expression of KIF1B and KIF5 that are molecular motors for neuronal anterograde traffic, in hippocampus, substantia nigra, and locus coeruleus of 10-month-old Lewis rats and cultured cells, from these same areas, following exposure to low doses of rotenone that do not lead to protein inclusions. The present study showed alteration in KIF1B and KIF5 expression, as well as in mitochondria mobility prior to protein aggregation involved in neurodegenerative disorders. These findings suggest that change in intracellular trafficking might be critical and one of the primary events for impairment of cell physiology during neurodegeneration associated with protein inclusions.
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Acknowledgments
The authors are grateful to Professors Luciana Amaral Haddad, Regina Celia Mingroni Netto, Angela Maria Vianna Morgante, and Luis Eduardo Soares Netto for their kind assistance in providing infrastructure to perform some of the experiments presented herein. This study was supported by research grants from Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP) (2008/04480-9; 2011/06434-7) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (472042/2008-4; 471779/2010-5). T.Q.M., S.A.M., and R.S.C. received scholarships from FAPESP (2009/12200-9; 2011/05576-2; 2011/00478-2, respectively); A.M.D. received scholarship from CNPq (PIBIC 124062/2010-5); and K.L.G.F. received a scholarship from Coordenacao de Aperfeicoamento de Pessoal de Nivel Superior (CAPES).
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Melo, T.Q., D’unhao, A.M., Martins, S.A. et al. Rotenone-Dependent Changes of Anterograde Motor Protein Expression and Mitochondrial Mobility in Brain Areas Related to Neurodegenerative Diseases. Cell Mol Neurobiol 33, 327–335 (2013). https://doi.org/10.1007/s10571-012-9898-z
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DOI: https://doi.org/10.1007/s10571-012-9898-z