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Thioredoxin-1 was Required for CREB Activity by Methamphetamine in Rat Pheochromocytoma Cells

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Abstract

Methamphetamine (METH) is one of the most commonly abused agents by illicit-drug users. Thioredoxin-1 (Trx-1) plays important biological roles both in intra- and extracellular compartments, including in regulation of various intracellular molecules via thiol redox control. In this study, we found that Trx-1 was induced by METH in rat pheochromocytoma PC12 cells. Furthermore, PI3K/Akt pathway was involved in METH-induced increase of Trx-1 expression. An increase in phosphorylated cAMP response element-binding protein (CREB) was also observed after exposure of PC12 cells to METH, which was inhibited by a PI3K inhibitor, LY294002. In addition, the siRNA targeted toTrx-1 reduced the level of phosphorylated CREB by METH, suggesting Trx-1 is necessary for increased activity of CREB by METH. The results obtained in this study showed that Trx-1 might play a role in the actions of METH.

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Acknowledgments

This study was supported by the National Natural Science Foundation of China (No. 81160162, U1202227), and a grant from the Key Laboratory of Medical Neurobiology, Kunming University of Science and Technology.

Conflict of interest

The authors declare that they have no competing financial interests.

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Authors and Affiliations

  1. Faculty of Environmental Science and Engineering, Kunming University of Science and Technology, Kunming, 650500, China

    Tao Lv & Sheng-Dong Wang

  2. Medical Faculty, Kunming University of Science and Technology, Kunming, 650500, China

    Tao Lv, Sheng-Dong Wang & Jie Bai

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  1. Tao Lv
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  2. Sheng-Dong Wang
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  3. Jie Bai
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Correspondence to Jie Bai.

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Lv, T., Wang, SD. & Bai, J. Thioredoxin-1 was Required for CREB Activity by Methamphetamine in Rat Pheochromocytoma Cells. Cell Mol Neurobiol 33, 319–325 (2013). https://doi.org/10.1007/s10571-012-9897-0

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  • DOI: https://doi.org/10.1007/s10571-012-9897-0

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