Abstract
In this study, we observed neuroblast differentiation in the somatosensory cortex (SSC) and hippocampal CA1 region (CA1), which is vulnerable to oxidative stress, of the mouse at various early postnatal days (P) 1, 7, 14, and 21 using doublecortin (DCX, a marker for neuroblasts). Cresyl violet and NeuN (Neuronal Nuclei) staining showed development of layers as well as neurons in the SSC and CA1. At P1, DCX-positive neuroblasts expressed strong DCX immunoreactivity in both the SSC and CA1. Thereafter, DCX immunoreactivity was decreased with time. At P7, many DCX-immunoreactive neuroblasts were well detected in the SSC and CA1. At P14, some DCX-positive neuroblasts were found in the SSC and CA1: The immunoreactivity was weak. At P21, DCX immunoreactivity was hardly found in cells in the SSC and CA1. These results suggest that DCX-positive neuroblasts were significantly decreased in the mouse SSC and CA1 from P14.
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Acknowledgments
The authors would like to thank Mr. Seung Uk Lee and Mrs. Hyun Sook Kim for their technical help and Seung-Hae Kwon of the Korean Basic Science Institute Chuncheon Center for technical assistance with the confocal image analyses. This study was supported by the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology (2010-0010580), and by a grant (2010K000823) from Brain Research Center of the 21st Century Frontier Research Program funded by the Ministry of Education, Science and Technology, the Republic of Korea.
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Dae Young Yoo and Ki-Yeon Yoo contributed equally to this article.
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Yoo, D.Y., Yoo, KY., Choi, J.W. et al. Time Course of Postnatal Distribution of Doublecortin Immunoreactive Developing/Maturing Neurons in the Somatosensory Cortex and Hippocampal CA1 Region of C57BL/6 Mice. Cell Mol Neurobiol 31, 729–736 (2011). https://doi.org/10.1007/s10571-011-9670-9
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DOI: https://doi.org/10.1007/s10571-011-9670-9