Abstract
This study was performed to determine whether minoxidil sulfate (MS), a selective Adenosine 5′-triphosphate-sensitive potassium channel (K ATP channel) activator, has an effect on the expression of caveolin-1 in the rat’s brain tumor tissue. Using a rat brain glioma (C6) model, we found that the expression of caveolin-1 protein at tumor sites was greatly increased after intracarotid infusion of MS at a dose of 30 μg/kg/min for 15, 30, and 60 min via Western blot analysis. And the peak value of the caveolin-1 expression was observed in rats with glioma after 15 min of MS perfusion, which was significantly attenuated by reactive oxygen species (ROS) scavenger (N-2-mercaptopropionyl glycine, MPG). In addition, MPG also significantly inhibited the increase of blood–brain tumor barrier (BTB) permeability which was induced by MS. This led to the conclusion that the MS-induced BTB permeability increase may be related to the accelerated formation of caveolin-1 protein, and could be mediated by ROS.
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Acknowledgments
This study was supported by doctor-beginning science foundation of Liaoning Province in China, No. 20101109, and special fund for scientific research of doctor-degree subject for the new teacher in colleges and universities in Department of Education in China, No. 20102134120007.
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Yan-ting Gu and Yi-xue Xue contributed equally to this study.
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Gu, Yt., Xue, Yx., Zhang, H. et al. Adenosine 5′-Triphosphate-Sensitive Potassium Channel Activator Induces the Up-Regulation of Caveolin-1 Expression in a Rat Brain Tumor Model. Cell Mol Neurobiol 31, 629–634 (2011). https://doi.org/10.1007/s10571-011-9658-5
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DOI: https://doi.org/10.1007/s10571-011-9658-5