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Expressions of Forkhead Class Box O 3a on Crushed Rat Sciatic Nerves and Differentiated Primary Schwann cells

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Abstract

Forkhead box-containing protein, class O 3 a (FOXO3a), an Akt downstream target, plays an important role in peripheral nervous system. FOXO3a shares the ability to be inhibited and translocated from the nucleus on phosphorylation by proteins such as Akt/PKB in the PI3K signaling pathway. To elucidate the expression and possible function of FOXO3a in lesion and repair, we performed an acute sciatic nerve crush model and studied differential expressions of FOXO3a. We observed that expressions of FOXO3a in Schwann cells (SCs) of the peripheral nervous system and cAMP-induced differentiation were dynamically regulated. Western blot analysis showed FOXO3a level significantly decreased post injury. Moreover, Immunofluorescence double labeling suggested the changes were striking especially in SCs. In vitro, Western blot analysis showed that the expression of FOXO3a was decreased in cAMP-induced differentiated primary SCs. The FOXO3a siRNA-transfected SCs treated by cAMP promote differentiation of SCs through the PI3K/Akt pathway. The results indicate that FOXO3a plays an important role during differentiation of SCs.

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Abbreviations

SCs:

Schwann cells

FOXO3a:

Forkhead class box O 3a

PI3-K:

Phosphatidylinositol 3-kinase

P0:

Myelin protein zero

cAMP:

Cyclic adenosine monophosphate

GAPDH:

Glyceraldehyde-3-phosphate dehydrogenase

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Acknowledgments

The authors wish to thank Dr. Yuxiang Hu for helpful criticism and linguistic revision of the manuscript. This work was supported by the National Natural Science Foundation of China (No. 30770488, No. 30870320, No. 31070723, and No. 81070275); Natural Science Foundation of Jiangsu province (No. BK2009156, No. BK2009157, and No. BK2010169).

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Authors and Affiliations

  1. Department of Orthopaedics, Affiliated Jiangyin Hospital of Nantong University, Nantong, China

    Qiuhong Wang, Youhua Wang, Zhengming Zhou, Xiang Lu, Yi Cao, Meijuan Yan, Yuhong Ji & Huiguang Yang

  2. Department of Immunology, Medical College, Nantong University, Nantong, 226001, China

    Qiuhong Wang, Yonghua Liu, Meijuan Yan, Fei He, Xia Pan, Xiaoli Qian & Yuhong Ji

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  1. Qiuhong Wang
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  2. Youhua Wang
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  3. Zhengming Zhou
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  4. Xiang Lu
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  6. Yonghua Liu
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Correspondence to Yuhong Ji or Huiguang Yang.

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First two authors contributed equally to this study.

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Wang, Q., Wang, Y., Zhou, Z. et al. Expressions of Forkhead Class Box O 3a on Crushed Rat Sciatic Nerves and Differentiated Primary Schwann cells. Cell Mol Neurobiol 31, 509–518 (2011). https://doi.org/10.1007/s10571-010-9644-3

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  • DOI: https://doi.org/10.1007/s10571-010-9644-3

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