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Age-related Changes in Calbindin-D28k, Parvalbumin, and Calretinin Immunoreactivity in the Dog Main Olfactory Bulb

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Abstract

Expression and age-related changes of calbindin-D28k (CB), parvalbumin (PV), and calretinin (CR) in the main olfactory bulb of the dog were investigated by immunohistochemistry and western blot analysis. Neurons that expressed these calcium-binding proteins showed a characteristic laminar distribution. Most of CB-immunoreactive neurons were observed in the glomerular layer (GL) and the inner sublayer of the external plexiform layer (EPL). Most of PV-immunoreactive neurons were observed in the outer sublayer of the EPL. CR-immunoreactive neurons were mainly distributed in the GL and the granule cell layer. With regard to age-related changes, CB-immunoreactive neurons in the GL were stable among all age groups; however, in the EPL they decreased with age. PV-immunoreactive neurons decreased in middle-aged and aged groups. However, CR-immunoreactive neurons were not decreased in middle-aged and aged groups. These results suggest that CB-immunoreactive neurons in the EPL were most sensitive to aging, and that their reduction may be related to aging in the dog.

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Acknowledgments

The authors would like to thank Mr. Seok Han, Mr. Seung Uk Lee, and Ms. Hyun Sook Kim for their technical help in this study. This work was supported by the Korea Research Foundation Grant funded by the Korean Government (MOEHRD, Basic Research Promotion Fund; KRF-2007-412-J00502).

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Authors and Affiliations

  1. Department of Anatomy and Neurobiology, Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University, Chuncheon, 200-702, South Korea

    Jung Hoon Choi, Choong Hyun Lee, Ki-Yeon Yoo & Moo-Ho Won

  2. Department of Anatomy and Cell Biology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University, Seoul, 151-742, South Korea

    In Koo Hwang & In Se Lee

  3. Department of Physiology, College of Medicine, Hallym University, Chuncheon, 200-702, South Korea

    Yun Lyul Lee & Hyung-Cheul Shin

  4. Institute of Natural Medicine, Hallym University, Chuncheon, 200-702, South Korea

    Jung Hoon Choi, Ki-Yeon Yoo, Hyung-Cheul Shin & Moo-Ho Won

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  1. Jung Hoon Choi
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Correspondence to In Se Lee or Moo-Ho Won.

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Choi, J.H., Lee, C.H., Yoo, KY. et al. Age-related Changes in Calbindin-D28k, Parvalbumin, and Calretinin Immunoreactivity in the Dog Main Olfactory Bulb. Cell Mol Neurobiol 30, 1–12 (2010). https://doi.org/10.1007/s10571-009-9425-z

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