Skip to main content

Advertisement

SpringerLink
Log in

Quantitation of O6-Methylguanine-DNA Methyltransferase Gene Messenger RNA in Gliomas by Means of Real-Time RT-PCR and Clinical Response to Nitrosoureas

  • Original Research
  • Published:
Cellular and Molecular Neurobiology Aims and scope Submit manuscript

Summary

  1. 1.

    O6-methylguanine-DNA methyltransferase (MGMT) mRNA was measured in 50 malignant gliomas that had received 1-(4-amino-2-methyl-5-pyrimidynyl) methyl-3-(2-chloroethyl)-3-nitrosourea hydrochloride (ACNU) after the resection of the tumor by real-time reverse transcription-polymerase chain reaction (RT-PCR) using TaqMan probe.

  2. 2.

    The mean absolute value of MGMTmRNA normalized to the level of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) for 50 tumors was 1.29 × 104± 1.28 × 104 copy/μg RNA (mean ± SD). The amount of MGMTmRNA less than 6 × 103 copy/μg RNA was the most significant factor in predicting the initial effect of treatment with ACNU by multi-variant regression analysis (p = 0.0157).

  3. 3.

    These results suggest that quantitation of MGMTmRNA is the excellent method for predicting for the effect of ACNU in glioma therapy.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aldea, C., Alvarez, C. P., Folgueira, L., Delgado, R., and Otero, J. R. (2002). Rapid detection of herpes simplex virus DNA in genital ulcers by real-time PCR using SYBR green I dye as the detection signal. J. Clin. Microbiol. 40:1060–1062.

    Article  PubMed  CAS  Google Scholar 

  • Arcicasa, M., Roncadin, M., Bortolus, R., Bassignano, G., Boz, G., Franchin, G., De Paoli, A., and Trovo, M. G. (1994). Results of three consecutive combined treatments for malignant gliomas. Ten-year experience at a single institution. Am. J. Clin. Oncol. 17:437–443.

    PubMed  CAS  Google Scholar 

  • Chomczynski, P., and Sacchi, N. (1987). Single-step methods of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction. Anal. Biochem. 162:156–159.

    Article  PubMed  CAS  Google Scholar 

  • Heid, C. A., Stevens, J., Livak, K. J., and Williams, P. M. (1996). Real time quantitative PCR. Genome Res. 6:986–994.

    PubMed  CAS  Google Scholar 

  • Mineura, K., Yanagisawa, T., Watanabe, K., Kowada, M., and Yasui, N. (1996). Human brain tumor O(6)-methylguanine-DNA methyltransferase mRNA and its significance as an indicator of selective chloroethylnitrosourea chemotherapy. Int. J. Cancer 69:420–425.

    Article  PubMed  CAS  Google Scholar 

  • Mullah, B., Livak, K., Andrus, A., and Kenney, P. (1998). Efficient synthesis of double dye-labeled oligodeoxyribonucleotide probes and their application in a real time PCR assay. Nucleic Acids Res. 26:1026–1031.

    Article  PubMed  CAS  Google Scholar 

  • Nagane, M., Shibui, S., Oyama, H., Asai, A., Kuchino, Y., and Nomura, K. (1995). Investigation of chemoresistance-related genes mRNA expression for selecting anticancer agents in successful adjuvant chemotherapy for a case of recurrent glioblastoma. Surg. Neurol. 44:462–468.

    PubMed  CAS  Google Scholar 

  • Saiki, R. K., Gelfand, D. H., Stoffel, S., Scharf, S. J., Higuchi, R., Horn, G. T., Mullis, K. B., and Erlich, H. A. (1988). Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491.

    PubMed  CAS  Google Scholar 

  • Silber, J. R., Mueller, B. A., Ewers, T. G., and Berger, M. S. (1993). Comparison of O6-methylguanine-DNA methyltransferase activity in brain tumors and adjacent normal brain. Cancer Res. 53:3416–3420.

    CAS  Google Scholar 

  • Tanaka, S., Kamitani, H., Amin, M. R., Watanabe, T., Oka, H., Fujii, K., Nagashima, T., and Hori, T. (2000). Preliminary individual adjuvant therapy for gliomas based on the results of molecular biological analyzes for drug-resistance genes. J. Neuro-Oncol. 46:157–171.

    Article  CAS  Google Scholar 

  • Tanaka, S., Kobayashi, I., Oka, H., Fujii, K., Watanabe, T., Nagashima, T., and Hori, T. (2003). O6-methylguanine-DNA methyltransferase gene expression in gliomas by quantitative real-time RT-PCR and clinical response to nitrosoureas. Int. J. Cancer 103:67–72.

    Article  PubMed  CAS  Google Scholar 

  • Tano, K., Shiota, S., Collier, J., Foote, R. S., and Mitra, S. (1990). Isolation and structural characterization of a cDNA clone encoding the human DNA repair protein for O6-alkylguanine. Proc. Natl. Acad. Sci. U.S.A. 87:686–690.

    PubMed  CAS  Google Scholar 

  • Yoshida, J., Kajita, Y., Wakabayashi, T., and Sugita, K. (1994). Long-term follow up results of 175 patients with malignant glioma: Importance of radical tumor resection and postoperative adjuvant therapy with interferon, ACNU and radiation. Acta. Neurochir. (Wien) 127:55–59.

    Article  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Neurosurgery, The Kitasato Institute Medical Center Hospital, 6-100 Arai, Kitamoto, Saitama, 364-8501, Japan

    Satoshi Tanaka

  2. Department of Neurosurgery, Kitasato University School of Medicine, Sagamihara, Japan

    Hidehiro Oka & Kiyotaka Fujii

  3. Gene and Chromosome Center, SRL Inc., Hino, Japan

    Kaoru Watanabe, Kumi Nagao & Atsushi Kakimoto

Authors
  1. Satoshi Tanaka
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Hidehiro Oka
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Kiyotaka Fujii
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Kaoru Watanabe
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Kumi Nagao
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Atsushi Kakimoto
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Satoshi Tanaka.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Tanaka, S., Oka, H., Fujii, K. et al. Quantitation of O6-Methylguanine-DNA Methyltransferase Gene Messenger RNA in Gliomas by Means of Real-Time RT-PCR and Clinical Response to Nitrosoureas. Cell Mol Neurobiol 25, 1067–1071 (2005). https://doi.org/10.1007/s10571-005-8475-0

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10571-005-8475-0

Key Words

Search

Navigation