Abstract
Cellulose nanocrystals (CNCs) and polydopamine (PDA) have shown great application potential in the biomedical field due to their excellent biocompatibility. Using PDA@CNC as a carrier to construct functional nanocomposites for targeted fluorescence imaging of tumor cells and effective cancer treatment can expand the applications of CNCs in tumor diagnosis and treatment. Here, we conveniently modified microRNA-21 inhibitors (miRNAi) on PDA@CNC to construct a multifunctional nanocomposite (FAM-miRNAi@PDA@CNC) for achieving in situ fluorescence imaging of tumor biomarkers and photothermal effects to induce death of breast cancer cells. The overexpressed miRNA-21 in breast cancer cells hybridizes with FAM-miRNAi, triggering green fluorescence of FAM to recognize breast cancer cells. The rod-shaped morphology of FAM-miRNAi@PDA@CNC can effectively promote the endocytosis of FAM-miRNAi@PDA@CNC by the cell membrane. In addition, CNCs can stabilize the photothermal performance of PDA, and the photothermal conversion efficiency of PDA@CNC reached up to 61.66%, which will help FAM-miRNAi@PDA@CNC to achieve 70% apoptosis rate of breast cancer cells under 808 nm laser irradiation. The design of FAM-miRNAi@PDA@CNC provides a valuable pathway for CNCs utilization in biomedicine and accurate diagnosis and treatment of breast cancer.
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Funding
This work was supported by the National Natural Science Foundation of China (21978069, and 22278112), Henan Province Science and Technology Research Project (222102520026), Postdoctoral Science Foundation of China (2022M711034), and Postdoctoral Research Grant in Henan Province (202102034).
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GH: Conceptualization, Data curation, and writing original draft. JN: Data analysis. TW: Experimental guidance. YL: Data curation. YN: Experimental guidance. JL: Formal analysis. YZ: Supervision, Revised the manuscript.
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Hu, G., Ning, J., Wu, T. et al. A multifunctional cellulose nanocomposite with targeted fluorescence imaging and photothermal effects on breast cancer cells. Cellulose 31, 3645–3656 (2024). https://doi.org/10.1007/s10570-024-05830-y
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DOI: https://doi.org/10.1007/s10570-024-05830-y