Abstract
The incorporation of hydroxyapatite (HA) into bacterial cellulose (BC) has become a promising approach in recent times to provide osteogenic abilities for BC scaffolds. In this study, gum tragacanth (GT) was added to the sugarcane juice media containing HA for bacterial cellulose cultivation to increase the attachment of the nanoparticles to cellulose fibers. The cellulose fibers experienced changes in fiber size and elemental compositions. The atomic % of Ca and P increased with increasing HA concentration in the BC culture media, with a significant amount higher in the GT sample. The viability of pre-osteoblast cells MC3T3 was higher in scaffolds with HA compared to the net BC. The alkaline phosphatase activities of the cells on the scaffolds during 21 days of cultivation confirmed the differentiation support of the BC/gum tragacanth/hydroxyapatite (BGA) scaffold. The use of colloidal media to attach nanoparticles on BC fibers is suggested as a promising approach to producing bioactive BC for biomedical applications.
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Acknowledgements
The authors would like to thank the National Research Council of Thailand (NRCT): NRCT5-RSA63001-06, Grant Number: NRCT-RSA63002-01 for financial support. We are grateful to the Department of Biotechnology, Faculty of Agro-Industry, Kasetsart University, Bangkok, Thailand for providing the laboratory equipment.
Funding
This study was funded by the National Research Council of Thailand for financial support (NRCT): NRCT5-RSA63001-06. Grant Number: NRCT-RSA63002-01.
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V.M. Quan: Conceived idea and designed the study, Acquired/analyzed data, and wrote the manuscript. T. Selorm: Prepare Figure 4, Revise the XRD discussion and edit the manuscript. N. Kamonsutthipaijit: Prepare Figure 4 and revise the XRD data presentation. P. Sukyai: Conceived idea, Acquire funding, Revised the manuscript and supervised the project. All authors reviewed and accepted the manuscript.
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Quan, V.M., Torgbo, S., Kamonsutthipaijit, N. et al. In situ preparation of bacterial cellulose/hydroxyapatite scaffold in colloidal culture media containing gum tragacanth. Cellulose 31, 1787–1800 (2024). https://doi.org/10.1007/s10570-023-05711-w
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DOI: https://doi.org/10.1007/s10570-023-05711-w