Abstract
Acidified aqueous solutions of different quaternary ammonium salts were tested for pretreating corn stover compared to control solutions, including single acidic solution and acidified NaCl and NH4Cl solutions. The results revealed that quaternary ammonium salt solutions were more effective in delignification and cellulose saccharification than the control solutions. The chemical structure of these salts significantly influenced the pretreatment efficacy. Notably, benzyltrimethylammonium chloride (BTMAC), a cost-effective option, exhibited remarkable capabilities. It achieved approximately 90% xylan removal, 86% lignin removal, and a 48-h enzymatic glucose yield of up to 93%. The BTMAC solution proved to be recycled for at least four runs while maintaining satisfactory performance. This work contributes valuable insights into the promising potential of standalone QAS solutions, offering a viable and cost-effective option for biomass pretreatment.
Similar content being viewed by others
Availability of data and materials
The datasets and materials used in the current study are available from the corresponding author on reasonable request.
Code availability
Not applicable.
References
Ahmed SF, Mofijur M, Chowdhury SN, Nahrin M, Rafa N, Chowdhury AT, Nuzhat S, Ong HC (2022) Pathways of lignocellulosic biomass deconstruction for biofuel and value-added products production. Fuel 318:123618. https://doi.org/10.1016/j.fuel.2022.123618
Bailey MJ, Biely P, Poutanen K (1992) Interlaboratory testing of methods for assay of xylanase activity. J Biotechnol 23(3):257–270. https://doi.org/10.1016/0168-1656(92)90074-J
Chen Z, Reznicek WD, Wan C (2018) Aqueous choline chloride: a novel solvent for switchgrass fractionation and subsequent hemicellulose conversion into furfural. ACS Sustain Chem Eng 6(5):6910–6919. https://doi.org/10.1021/acssuschemeng.8b00728
Chen Y-L, Zhang X, You T-T, Xu F (2019) Deep eutectic solvents (DESs) for cellulose dissolution: a mini-review. Cellulose 26(1):205–213. https://doi.org/10.1007/s10570-018-2130-7
Chen Z, Wang Y, Cheng H, Zhou H (2022) Hemicellulose degradation: an overlooked issue in acidic deep eutectic solvents pretreatment of lignocellulosic biomass. Ind Crops Prod 187:115335. https://doi.org/10.1016/j.indcrop.2022.115335
French AD (2014) Idealized powder diffraction patterns for cellulose polymorphs. Cellulose 21(2):885–896. https://doi.org/10.1007/s10570-013-0030-4
Ghose T (1987) Measurement of cellulase activities. Pure Appl Chem 59(2):257–268. https://doi.org/10.1351/pac198759020257
Guo Z, Zhang Q, You T, Zhang X, Xu F, Wu Y (2019) Short-time deep eutectic solvent pretreatment for enhanced enzymatic saccharification and lignin valorization. Green Chem 21(11):3099–3108. https://doi.org/10.1039/C9GC00704K
Himmel ME, Ding S-Y, Johnson DK, Adney WS, Nimlos MR, Brady JW, Foust TD (2007) Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science 315(5813):804–807. https://doi.org/10.1126/science.1137016
Karimi K, Taherzadeh MJ (2016) A critical review of analytical methods in pretreatment of lignocelluloses: composition, imaging, and crystallinity. Bioresour Technol 200:1008–1018. https://doi.org/10.1016/j.biortech.2015.11.022
Kumar B, Bhardwaj N, Agrawal K, Chaturvedi V, Verma P (2020a) Current perspective on pretreatment technologies using lignocellulosic biomass: An emerging biorefinery concept. Fuel Process Technol 199:106244. https://doi.org/10.1016/j.fuproc.2019.106244
Kumar V, Yadav SK, Kumar J, Ahluwalia V (2020b) A critical review on current strategies and trends employed for removal of inhibitors and toxic materials generated during biomass pretreatment. Bioresour Technol 299:122633. https://doi.org/10.1016/j.biortech.2019.122633
Kurian JK, Gariepy Y, Orsat V, Raghavan GSV (2015) Comparison of steam-assisted versus microwave-assisted treatments for the fractionation of sweet sorghum bagasse. Bioresour Bioprocess 2(1):30. https://doi.org/10.1186/s40643-015-0059-3
Li Y, Qi B, Wan Y (2020) Separation of monosaccharides from pretreatment inhibitors by nanofiltration in lignocellulosic hydrolysate: fouling mitigation by activated carbon adsorption. Biomass Bioenerg 136:105527. https://doi.org/10.1016/j.biombioe.2020.105527
Liang X, Zhu Y, Qi B, Li S, Luo J, Wan Y (2021) Structure-property-performance relationships of lactic acid-based deep eutectic solvents with different hydrogen bond acceptors for corn stover pretreatment. Bioresour Technol 336:125312. https://doi.org/10.1016/j.biortech.2021.125312
Lorenci Woiciechowski A, Dalmas Neto CJ, de Souza P, Vandenberghe L, de Carvalho Neto DP, Novak Sydney AC, Letti LAJ, Karp SG, Zevallos Torres LA, Soccol CR (2020) Lignocellulosic biomass: acid and alkaline pretreatments and their effects on biomass recalcitrance—conventional processing and recent advances. Bioresour Technol 304:122848. https://doi.org/10.1016/j.biortech.2020.122848
Qi B, Wang Z, Luo J, Wan Y (2021) Pretreatment of corn stover by acidic and basic choline chloride solutions for enzymatic hydrolysis. Cellulose 28(16):10127–10141. https://doi.org/10.1007/s10570-021-04163-4
Sankaran R, Parra Cruz RA, Pakalapati H, Show PL, Ling TC, Chen W-H, Tao Y (2020) Recent advances in the pretreatment of microalgal and lignocellulosic biomass: a comprehensive review. Bioresour Technol 298:122476. https://doi.org/10.1016/j.biortech.2019.122476
Segal L, Creely J, Martin A Jr, Conrad C (1959) An empirical method for estimating the degree of crystallinity of native cellulose using the X-ray diffractometer. Text Res J 29(10):786–794. https://doi.org/10.1177/004051755902901003
Sheng Y, Lam SS, Wu Y, Ge S, Wu J, Cai L, Huang Z, Le QV, Sonne C, Xia C (2021) Enzymatic conversion of pretreated lignocellulosic biomass: a review on influence of structural changes of lignin. Bioresour Technol 324:124631. https://doi.org/10.1016/j.biortech.2020.124631
Shinde SD, Meng X, Kumar R, Ragauskas AJ (2018) Recent advances in understanding the pseudo-lignin formation in a lignocellulosic biorefinery. Green Chem 20(10):2192–2205. https://doi.org/10.1039/C8GC00353J
Sidana A, Yadav SK (2022) Recent developments in lignocellulosic biomass pretreatment with a focus on eco-friendly, non-conventional methods. J Clean Prod 335:130286. https://doi.org/10.1016/j.jclepro.2021.130286
Sluiter A, Hames B, Scarlata C, Sluiter J, Templeton D, Crocker D (2008) Determination of structural carbohydrates and lignin in biomass (NREL/TP-510-42618). Laboratory Analytical Procedure (LAP). National Renewable Energy Lab.(NREL), Golden, CO (United States)
Tan YT, Chua ASM, Ngoh GC (2020) Deep eutectic solvent for lignocellulosic biomass fractionation and the subsequent conversion to bio-based products—a review. Bioresour Technol 297:122522. https://doi.org/10.1016/j.biortech.2019.122522
Tarasov D, Leitch M, Fatehi P (2018) Lignin–carbohydrate complexes: properties, applications, analyses, and methods of extraction: a review. Biotechnol Biofuels 11(1):269. https://doi.org/10.1186/s13068-018-1262-1
Wang W, Lee D-J (2021) Lignocellulosic biomass pretreatment by deep eutectic solvents on lignin extraction and saccharification enhancement: a review. Bioresour Technol 339:125587. https://doi.org/10.1016/j.biortech.2021.125587
Yao L, Yang H, Meng X, Ragauskas AJ (2022) Toward a fundamental understanding of the role of lignin in the biorefinery process. Front Energy Rese. https://doi.org/10.3389/fenrg.2021.804086
Yiin CL, Yap KL, Ku AZE, Chin BLF, Lock SSM, Cheah KW, Loy ACM, Chan YH (2021) Recent advances in green solvents for lignocellulosic biomass pretreatment: potential of choline chloride (ChCl) based solvents. Bioresour Technol 333:125195. https://doi.org/10.1016/j.biortech.2021.125195
Yoo CG, Meng X, Pu Y, Ragauskas AJ (2020) The critical role of lignin in lignocellulosic biomass conversion and recent pretreatment strategies: a comprehensive review. Bioresour Technol 301:122784. https://doi.org/10.1016/j.biortech.2020.122784
Yuan Y, Jiang B, Chen H, Wu W, Wu S, Jin Y, Xiao H (2021) Recent advances in understanding the effects of lignin structural characteristics on enzymatic hydrolysis. Biotechnol Biofuels 14(1):205. https://doi.org/10.1186/s13068-021-02054-1
Zhang H, Han L, Dong H (2021) An insight to pretreatment, enzyme adsorption and enzymatic hydrolysis of lignocellulosic biomass: experimental and modeling studies. Renew Sustain Energy Rev 140:110758. https://doi.org/10.1016/j.rser.2021.110758
Zhao X, Zhang L, Liu D (2012) Biomass recalcitrance. Part I: the chemical compositions and physical structures affecting the enzymatic hydrolysis of lignocellulose. Biofuels Bioprod Biorefining 6(4):465–482. https://doi.org/10.1002/bbb.1331
Zhu Y, Qi B, Liang X, Luo J, Wan Y (2021) Lewis acid-mediated aqueous glycerol pretreatment of sugarcane bagasse: pretreatment recycling, one-pot hydrolysis and lignin properties. Renew Energy 178:1456–1465. https://doi.org/10.1016/j.renene.2021.07.006
Funding
This work was supported by the National Key R&D Program of China (Grant No. 2019YFC1906601).
Author information
Authors and Affiliations
Contributions
Benkun Qi conceived and designed the study, conducted the data analysis, and wrote the first draf of the manuscript. Yuan Zhu and Liyi Zhang performed the experiments. Jianquan Luo and Yinhua Wan critically reviewed the manuscript. All the authors read and approved the final mauscript.
Corresponding author
Ethics declarations
Conflict of interest
The authors declare that they have no competing interests.
Ethical approval
Not applicable.
Consent to participate
Not applicable.
Consent for publication
Not applicable.
Additional information
Publisher's Note
Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.
Supplementary Information
Below is the link to the electronic supplementary material.
Rights and permissions
Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law.
About this article
Cite this article
Zhu, Y., Zhang, L., Qi, B. et al. Pretreatment of corn stover using aqueous quaternary ammonium salt solutions for improved enzymatic saccharification. Cellulose 31, 1519–1532 (2024). https://doi.org/10.1007/s10570-023-05710-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10570-023-05710-x