Abstract
In the presented study, thymol-containing wound dressing films were prepared in the presence of polysaccharide gellan gum, carboxymethyl cellulose and hyaluronic acid polymers by solvent casting technique. The structure of the obtained films was characterized by Fourier transform infrared attenuated total reflection spectroscopy and scanning electron microscopy analysis. The swelling ratios of the thymol-containing films were adequate to supply a moist environment (∼ 829%, for 24 h measurement) and underwent a hydrolytic degradation (∼ 55%, for 21 days measurement). The water vapor transmission rates were moderate for sufficient moisture (∼ 2376 g/m2/day). According to the findings, the addition of thymol to the polymeric films increased the elongation at break value. The cumulative release amounts of thymol were increased from 4.42 ± 0.40 mg thymol/g film to 6.25 ± 0.39 mg thymol/g film by increasing thymol concentration in the formulation. In vitro biocompatibility and wound healing tests were also performed against the L929 fibroblast cell line. The findings demonstrated that the migration and proliferation of L929 fibroblast cells were greatly improved by the formulation of non-toxic polysaccharide-based 5% (w) thymol-containing film (GCH-5), and therefore it could be a potential wound dressing material for the clinical use.
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Data availability
The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgments
The authors are grateful for the support of Assoc. Prof. Ayça Bal Öztürk with mechanical tests.
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This work was supported by the Çankırı Karatekin University Scientific Research Projects Coordination Office (Grant Number: MF211221L23).
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All authors contributed to the study’s conception and design. Formal analysis and investigation: G.A. and B.Ö., Data collection and analysis: G.A. and B.Ö., Writing-first draft preparation: B.Ö. and Z.Ö., Writing-review and editing: Z.Ö., Supervision: Z.Ö.
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Acar, G., Özkahraman, B. & Özbaş, Z. Thymol incorporated gellan gum/carboxymethyl cellulose/hyaluronic acid films for wound dressings applications. Cellulose 30, 9517–9528 (2023). https://doi.org/10.1007/s10570-023-05451-x
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DOI: https://doi.org/10.1007/s10570-023-05451-x