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Yield and quality of bacterial cellulose from agricultural waste

Abstract

The global abundance and availability of oat hulls make them a promising feedstock to produce a unique type of cellulose, the bacterial one. This is the first study examining how a chemical pretreatment method of oat hulls influences the yield and properties of bacterial cellulose (BC) in extended cultivation. Here we employed our own pretreatment methods that use dilute HNO3 and NaOH solutions in one and two stages, a total of four pretreatment methods. Further technological stages were performed in the same manner: pulps were enzymatically hydrolyzed with commercial enzymes CelloLux-A and BrewZyme BGX, and biosynthesis of BC was run using the Medusomyces gisevii Sa-12 symbiotic culture. A two-stage (HNO3 + NaOH) pretreatment of oat hulls was found to afford a biologically good medium and increase the BC yield 1.8−3.2-fold compared to the other pretreatments used. A pretreatment method of oat hulls determined the BC yield and degree of polymerization. However, a pretreatment method had no impact on the highest crystallinity index and allomorph Iα content of all the BC samples, which is explained by Medusomyces gisevii Sa-12 used. The crystallinity index and allomorph Iα content, as measured by X-ray diffractometry, are proposed for use as BC quality assessment criteria.

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Acknowledgments

Funding was provided by Russian Science Foundation (Grant No. 17-19-01054).

Funding

The research was supported by the Russian Science Foundation (Grant #17–19-01054).

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EAS Conceptualization, Investigation, Writing—original draft, Writing—review & editing, Visualization, Validation. EKG Investigation, Writing—original draft, Visualization, Formal analysis. VVB Conceptualization, Investigation, Writing—review & editing, Validation. LAA Formal analysis, Writing—review & editing. GVS Supervision.

Corresponding author

Correspondence to Vera V. Budaeva.

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The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.

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This article does not contain any studies with human participants or animals performed by any of the authors.

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Skiba, E.A., Gladysheva, E.K., Budaeva, V.V. et al. Yield and quality of bacterial cellulose from agricultural waste. Cellulose 29, 1543–1555 (2022). https://doi.org/10.1007/s10570-021-04372-x

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  • DOI: https://doi.org/10.1007/s10570-021-04372-x

Keywords

  • Oat hulls
  • Lignocellulose pretreatment
  • Bacterial cellulose
  • Medusomyces gisevii