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Influences of polysaccharides in wood cell walls on lignification in vitro

Abstract

To elucidate the effects of polysaccharides, cellulose, water-soluble xylan (WXY), galactoglucomannan (GGM) and xyloglucan (XG) on lignification in vitro, artificial polysaccharide matrices were prepared from a combination of cellulose and hemicelluloses, and dehydrogenation polymer (DHP) was synthesized from coniferyl alcohol in the presence of the matrices by using horseradish peroxidase (HRP). Prior to DHP formation, interactions between cellulose and hemicelluloses were investigated with equilibrium adsorptions of the hemicelluloses on bacterial cellulose (BC) films and with quartz crystal microbalance with dissipation technique (QCM-D) to determine their adsorption on cellulose nanofibers (CNFs). Both analyses showed that the order of adsorption amounts was XG > GGM > WXY. The QCM-D experiments also suggested that HRP strongly interacted with cellulose rather than hemicelluloses. The amount of DHP generated in the XG-BC matrix was the largest among the prepared matrices, and XG facilitated the formation of 5–5′ interunitary linkages. Thus, XG must be involved in the lignification in primary wood cell wall. On the other hand, the amount of DHP in the GGM-BC matrix was the smallest, indicating that GGM hampered lignification.

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Acknowledgments

This study was financial supported by JSPS KAKENHI (Grant-in-Aid for Scientific Research (A)) Grant Numbers 26252022 and 18H03954. We are thankful to the China Scholarship Council to provide a scholarship for Yan Lyu.

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Correspondence to Yasumitsu Uraki.

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We followed ethical standards; no animal and human participants were involved in the study.

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Lyu, Y., Matsumoto, T., Taira, S. et al. Influences of polysaccharides in wood cell walls on lignification in vitro. Cellulose 28, 9907–9917 (2021). https://doi.org/10.1007/s10570-021-04108-x

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  • DOI: https://doi.org/10.1007/s10570-021-04108-x

Keywords

  • Cellulose
  • Xylan
  • Galactoglucomannan
  • Xyloglucan
  • Aryl ether bond
  • 5–5′ interunitary bond