Abstract
The three-phase structure of the solid fermentation substrates is composed by the solid, liquid and gas phases, adjusting the ratio of the three-phase structure has proven to be a strategy that can better serve the growth of microorganisms. In this study, the effect of the three-phase structure of the cultivated substrate on the growth of Lentinula edodes was evaluated. Our results showed that the liquid phase was most impacted by the particle size and moisture content among the three-phase. Further analysis of the three-phase structure index (TPSI) showed that the highest efficiency of L. edodes growth was achieved at TPSI value of 23.3, and the liquid phase was the most affected variable on the TPSI of cultivated substrates among the three phases. Further, to determine the optimal water environment for L. edodes in the substrates, three types of water containing four proton components in the cultivation substrate were identified based on distributed exponential curve fitting of transverse relaxation (T2), namely, bound water T2b, free water T21a and T21b, and bulk water T22. L. edodes exhibited the best growth efficiency on elm (59% moisture content), peanut shell (52% moisture content) and apricot (56% moisture content) substrates, all of which were filled with free water and without bulk water, respectively. The same conclusion was also obtained in the mixed material cultivation substrate. In overview, L. edodes had the best growth efficiency in substrates filled with free water and not containing bulk water, which enabled proton analysis based on LF-NMR spectroscopy to provide new insights into the moisture content of wood-rot fungal cultivated substrates.
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This work was financially supported by the National Key R&D Program of China (No. 2019YFD1001900), the China Agriculture Research System of MOF and MARA (No. CARS20), the Fundamental Research Funds for the Central Universities (No. KYYZ202002), and the Key Research and Development Program of Ningxia Hui Autonomous Region, P. R. China (No. 2019BBF02032).
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YW designed the experiments and was a major contributor in data analysis and writing the manuscript. SH and ZY partly analyzed the data and were a major contributor in conducting the experiments. QJ partly analyzed the data and wrote the manuscript. AR, HY and MZ financed the research. QH helped YW conduct part of experiments. All authors read and approved the final manuscript.
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Wang, Y., Hu, S., Yang, Z. et al. Three-phase structure analysis of sawdust cultivation substrates and rapid detection of the optimal water environment for Lentinula edodes growth based on LF-NMR. Cellulose 28, 6917–6933 (2021). https://doi.org/10.1007/s10570-021-03987-4
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DOI: https://doi.org/10.1007/s10570-021-03987-4