Bacterial nanocellulose membrane as bolus in radiotherapy: "proof of concept"


Boluses characterize materials with an electromagnetic radiation attenuation coefficient similar to biological tissue and used to restrict the penetration of high energy photons and electrons used in radiotherapy for the treatment of superficial tumors. The development of new materials, mainly from sustainable biotechnological routes, will contribute to increase efficiency and expand the use of these technologies. The objective of this research was to develop the “proof of concept” regarding the use of the bacterial nanocellulose membrane (BNCm) as a bolus. For this purpose, BNCm were produced, purified and subjected to the physical–chemical characterization. The radiological density (RD) and radiation attenuation potential (RAP) of the BNCm were established and compared to a commercial bolus (CB). The moldability of BNCm was established and compared to the virtual bolus of dosimetric planning. The physical–chemical analysis demonstrated the constitution of a pure, highly hydrated, homogeneous and nanostructured network of cellulose fibers. BNCm showed superiority in relation to RD and similar RAP values when compared to CB. Moldability analysis showed a profile identical to a virtual bolus. The results validate the concept of using BNCm as a highly efficient biotechnological device, aligned with the idea of sustainability, as a bolus for use in radiotherapy.

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  1. Barud HS, Regiani T, Marques RFC, Lustri WR, Messaddeq Y, Ribeiro SJL (2011) Antimicrobial bacterial cellulose-silver nanoparticles composite membranes. J Nanomater 2011:1–8.

    CAS  Article  Google Scholar 

  2. Barud HS, De Araújo Júnior AM, Saska S, Mestieri LB, Campos JADB, Freitas RM, Ferreira NU, Nascimento AP, Miguel FG, Vaz MMOLL, Barizon EA, Oliveira FM, Gaspar AMM, Ribeiro SJL, Berretta AA (2013) Antimicrobial Brazilian propolis (EPP-AF) containing biocellulose membranes as promising biomaterial for skin wound healing. Evid Based Complement Altern Med 2013:1–10.

    Article  Google Scholar 

  3. Bray F, Ferlay J, Soerjomataram I, Siegel RL, Torre LA, Jemal A (2018) Global cancer statistics 2018: GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries. CA A Cancer J Clin 68:394–424.

    CAS  Article  Google Scholar 

  4. Butchosa N, Brown C, Larsson PT, Berglund LA, Bulone V, Zhou Q (2013) Nanocomposites of bacterial cellulose nanofibers and chitin nanocrystals: Fabrication, characterization and bactericidal activity. Green chem 15(12):3404–3413.

    CAS  Article  Google Scholar 

  5. Chummun S, McLean NR (2017) The management of malignant skin cancers. Surgery (United Kingdom) 35:519–524.

    Article  Google Scholar 

  6. Craythorne E, Al-Niami F (2017) Skin cancer. Medicine (United Kingdom) 45:431–434.

    Article  Google Scholar 

  7. de Oliveira Barud HG, Barud HDS, Cavicchioli M, Amaral TS, Oliveira Junior OB, Santos DM, Petersen ALOA, Celes F, Borges VM, Oliveira CI, Oliveira PF, Furtado RA, Tavares DC, Ribeiro SJL (2015) Preparation and characterization of a bacterial cellulose/silk fibroin sponge scaffold for tissue regeneration. Carbohydr Polym 128:41–51.

    CAS  Article  PubMed  Google Scholar 

  8. de Oliveira Barud HG, da Silva RR, da Silva BH, Tercjak A, Gutierrez J, Lustri WR, de Oliveira Junior OB, Ribeiro SJL (2016) A multipurpose natural and renewable polymer in medical applications: bacterial cellulose. Carbohydr Polym 153:406–420.

    CAS  Article  PubMed  Google Scholar 

  9. De Salvi DTB, Barud HS, Caiut JMA, Messaddeq Y, Ribeiro SJL (2012) Self-supported bacterial cellulose/boehmite organic–inorganic hybrid films. J Sol-Gel Sci Technol 63:211–218.

    CAS  Article  Google Scholar 

  10. Dos Santos RAC, Berretta AA, Barud HS, Ribeiro SJL, González-Garcia LN, Zucchi TD, Goldman GH, Riaño-Pachón DM (2014) Draft Genome Sequence of Komagataeibacter rhaeticus Strain AF1, a High Producer of Cellulose. Isol Kombucha Tea 2:e00731-e814.

    Article  Google Scholar 

  11. Fujimoto K, Shiinoki T, Yuasa Y, Hanazawa H, Shibuya K (2017) Efficacy of patient-specific bolus created using three-dimensional printing technique in photon radiotherapy. Phys Med 38:1–9.

    Article  PubMed  Google Scholar 

  12. Islam S, Mahmoud KA, Sayyed MI, Alim B, Rahman MdM, Mollah AS (2019) Study on the radiation attenuation properties of locally available bees-wax as a tissue equivalent bolus material in radiotherapy. Radiat Phys Chem 172:108559.

    CAS  Article  Google Scholar 

  13. Marins JA, Soares BG, Dahmouch K, Ribeiro SJL, Barud H, Bonemer D (2011) Structure and properties of conducting bacterial cellulose-polyaniline nanocomposites. Cellulose 18:1285–1294.

    CAS  Article  Google Scholar 

  14. Martins D, de Carvalho FD, Gama M, Dourado F (2020) Dry bacterial cellulose and carboxymethyl cellulose formulations with interfacial-activ performance: processing conditions and redispersion. Cellulose.

    Article  Google Scholar 

  15. Mierzwa ML (2019) Radiotherapy for Skin Cancers of the Face, Head, and Neck. Fac Plast Surg Clin North Am 27:131–138.

    Article  Google Scholar 

  16. Pang M, Huang Y, Meng F, Zhuang Y, Liu H, Du M, Ma Q, Wang Q, Chen Z, Chen L, Cai T, Cai Y (2019) Application of bacterial cellulose in skin and bone tissue engineering. Eur Polym J 122:109365.

    CAS  Article  Google Scholar 

  17. Tanskul S, Amornthatree K, Jaturonlak N (2013) A new cellulose-producing bacterium, Rhodococcus sp. MI 2: screening and optimization of culture conditions. Carb Pol 92:421–428.

    CAS  Article  Google Scholar 

  18. Vyas V, Palmer L, Mudge R, Jiang R, Fleck A, Schaly B, Osei E, Charland P (2013) On bolus for megavoltage photon and electron radiation therapy. Med Dosim 38:268–273.

    Article  PubMed  Google Scholar 

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We thank the company Senven Biotechnology for supplying the inputs for the production of nanocellulose membranes and the Radiology Center of Hospital Santa Casa de Misericórdia da Araraquara for carrying out the computed tomography analyzes. The authors also thank Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq, São Paulo Research Foundation (FAPESP), and TA Instruments Brasil.


The authors thank Conselho Nacional de Desenvolvimento Científico e Tecnológico—CNPq [grant number; 407822/2018–6; INCT-INFO] and São Paulo Research Foundation (FAPESP) [CEPID–13/ 07276–1 and 2018/25512–8].

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GCC: Conceptualization, methodology and writing—original draft. GPM: Conceptualization, methodology, investigation and writing—review & editing. FPV: Methodology and investigation. AMC: Investigation, methodology and resources. CSTA: Methodology, investigation and formal analysis. NCA: Investigation, methodology and resources. HSB: Investigation, methodology, supervision and writing—review & editing. ACA: Conceptualization, methodology, project administration, supervision and writing—review & editing.

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Correspondence to André Capaldo Amaral.

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Chiozzini, G.C., Mendes, G.P., Vanni, F.P. et al. Bacterial nanocellulose membrane as bolus in radiotherapy: "proof of concept". Cellulose (2020).

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  • Bacterial nanocellulose
  • Cellulose
  • Medical biotechnology
  • Radiotherapy
  • Bolus
  • Superficial tumors