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Bio-inspired biopolymeric coacervation for entrapment and targeted release of anthocyanin

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Abstract

In order to provide stability of anthocyanin, a powerful antioxidant, encapsulant was made by utilizing two biopolymers that can be used further for the targeted release due to its intrinsic structure. Present study was carried out to encapsulate anthocyanin in coacervate employing pectin and chitosan. Entrapment efficiency of anthocyanin was evaluated in coacervates prepared in nine different combinations employing three concentration each of pectin (40, 50 and 60 mg mL−1) and chitosan (5, 6 and 7 mg mL−1) in presence of CaCl2. The developed formulation was characterised by FT-IR, XRD and SEM techniques, which clearly depicts ionic interaction between chitosan and pectin. Release pattern of anthocyanin from the coacervate followed Korsmeyer-Peppas model. The n-values calculated from the curves suggested a quasi-Fickian diffusion pattern pH 1.7 and 4.0. However, at pH 7.0, the behaviour of release was presumed to be an anomalous or case-II transport. In-vitro study revealed that bioaccessibility (%) of coacervated formulation (24.3 ± 0.5) was significantly higher than crude extract (12.2 ± 1.0) as well as purified anthocyanin crystals (18.9 ± 0.7). Better bioaccessibility of coacervated anthocyanin provided important information about its potential use in functional foods.

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References

  • Al-Azi SOSM, Tan YTF, Wong TW (2014) Transforming large molecular weight pectin and chitosan into oral protein drug nanoparticulate carrier. React Funct Polym 84:45–52

    Article  Google Scholar 

  • Andishmand H, Tabibiazar M, Mohammadifar MA et al (2017) Pectin-zinc-chitosan-polyethylene glycol colloidal nano-suspension as a food grade carrier for colon targeted delivery of resveratrol. Int J Biol Macromol 97:16–22

    Article  CAS  Google Scholar 

  • Bernabé P, Peniche C, Argüelles-Monal W (2005) Swelling behavior of chitosan/pectin polyelectrolyte complex membranes. Effect of thermal cross-linking. Polymer Bull 55:367–375

    Article  Google Scholar 

  • Bigucci F, Luppi B, Cerchiara T et al (2008) Chitosan/pectin polyelectrolyte complexes: selection of suitable preparative conditions for colon-specific delivery of vancomycin. Eur J Pharm Sci 35:435–441

    Article  CAS  Google Scholar 

  • Bouayed J, Hoffmann L, Bohn T (2011) Total phenolics, flavonoids, anthocyanins and antioxidant activity following simulated gastro-intestinal digestion and dialysis of apple varieties: bioaccessibility and potential uptake. Food Chem 128:14–21

    Article  CAS  Google Scholar 

  • Boyapally H, Nukala RK, Bhujbal P et al (2010) Controlled release from directly compressible theophylline buccal tablets. Coll Surf B Biointerfaces 77(2):227–233

    Article  CAS  Google Scholar 

  • Carrillo C, Buvé C, Panozzo A et al (2017) Role of structural barriers in the in vitro bioaccessibility of anthocyanins in comparison with carotenoids. Food Chem 227:271–279

    Article  CAS  Google Scholar 

  • Comunian TA, Thomazini M, Alves AJG et al (2013) Microencapsulation of ascorbic acid by complex coacervation: protection and controlled release. Food Res Int 52:373–379

    Article  CAS  Google Scholar 

  • Dall'Asta M, Calani L, Tedeschi M et al (2012) Identification of microbial metabolites derived from in vitro fecal fermentation of different polyphenolic food sources. Nutrition 28(2):197–203

    Article  CAS  Google Scholar 

  • de Souza JRR, de Carvalho JIX, Trevisan MTS et al (2009) Chitosan-coated pectin beads: characterization and in vitro release of mangiferin. Food Hydrocoll 23:2278–2286

    Article  Google Scholar 

  • De Yao K, Liu J, Cheng GX et al (1996) Swelling behavior of pectin/chitosan complex films. J Appl Polym Sci 60:279–283

    Article  CAS  Google Scholar 

  • Fernández-Hervása MJ, Fellb JT (1998) Pectin/chitosan mixtures as coatings for colon-specific drug delivery: an in vitro evaluation. Int J Pharm 169:115–119

    Article  Google Scholar 

  • Ghaffari A, Navaee K, Oskoui M et al ( (2007) Preparation and characterization of free mixed-film of pectin/chitosan/Eudragit®RS intended for sigmoidal drug delivery. Eur J Pharm Biopharm 67:175–186 )

    Article  CAS  Google Scholar 

  • Gil-Izquierdo A, Zafrilla P, Tomás-Barberán (2002) An in vitro method to simulate phenolic compound release from the food matrix in the gastrointestinal tract. Eur Food Res Tech 214:155–159

    Article  CAS  Google Scholar 

  • Huang GQ, Liu LN, Han XN et al (2017) Intestine-targeted delivery potency of the O-carboxymethyl chitosan–gum Arabic coacervate: Effects of coacervation acidity and possible mechanism. Materials Sci Eng C 79:423–429

    Article  CAS  Google Scholar 

  • Kamiloglu S, Capanoglu E, Grootaert C et al (2015) Anthocyanin absorption and metabolism by human intestinal Caco-2 cells—a review. Int J Mol Sci 16:21555–21574

    Article  CAS  Google Scholar 

  • Kim TH, Park YH, Kim KJ et al (2003) Release of albumin from chitosan-coated pectin beads in vitro. Int J Pharm 250:371–383

    Article  CAS  Google Scholar 

  • Konczak I, Zhang W (2004) Anthocyanins—more than nature’s colours. J Biomed Biotech 239–240

  • Kurukji D, Norton I, Spyropoulos F (2016) Fabrication of sub-micron protein-chitosan electrostatic complexes for encapsulation and pH-modulated delivery of model hydrophilic active compounds. Food Hydrocoll 53:249–260

    Article  CAS  Google Scholar 

  • Liang L, Wu X, Zhao T et al (2012) In vitro bioaccessibility and antioxidant activity of anthocyanins from mulberry (Morus atropurpurea Roxb.) following simulated gastro-intestinal digestion. Food Res Int 46:76–82

    Article  CAS  Google Scholar 

  • McDougall GJ, Dobson P, Smith P et al (2005) Assessing potential bioavailability of raspberry anthocyanins using an in vitro digestion system. J Agric Food Chem 53:5896–5904

    Article  CAS  Google Scholar 

  • Mülleder U, Murkovic M, Pfannhauser W (2002) Urinary excretion of cyanidin glycosides. J Biochem Biophys Methods 53:61–66

    Article  Google Scholar 

  • Naidu VGM, Madhusudhana K, Sashidhar RB et al (2009) Polyelectrolyte complexes of gum kondagogu and chitosan, as diclofenac carriers. Carbohydr Polym 76(3):464–471

    Article  CAS  Google Scholar 

  • Nori MP, Favaro-Trindade CS, Matias de Alencar S et al (2011) Microencapsulation of propolis extract by complex coacervation. LWT-Food Sci Technol 44:429–435

    Article  CAS  Google Scholar 

  • Oidtmann J, Schantz M, Mäder K et al (2012) Preparation and comparative release characteristics of three anthocyanin encapsulation systems. J Agric Food Chem 60:844–851

    Article  CAS  Google Scholar 

  • Pandey S, Mishra A, Raval P et al (2013) Chitosan-pectin polyelectrolyte complex as a carrier for colon targeted drug delivery. J Young Pharm 5:160–166

    Article  CAS  Google Scholar 

  • Saha S, Singh J, Paul A et al (2020) Anthocyanin Profiling Using UV-Vis Spectroscopy and Liquid Chromatography Mass Spectrometry. J AOAC Int 103(1):23–39

    Article  Google Scholar 

  • Sarkar R, Kundu A, Banerjee K et al (2018) Anthocyanin composition and potential bioactivity of karonda (Carissa carandas) L. fruit: an Indian source of biocolorant. LWT-Food Sci Technol 93:673–678

    Article  CAS  Google Scholar 

  • Shaddel R, Hesari J, Azadmard-Damirchi S et al (2018) Double emulsion followed by complex coacervation as a promising method for protection of black raspberry anthocyanins. Food Hydrocoll 77:803–816

    Article  CAS  Google Scholar 

  • Silva DF, Favaro-Trindade CS, Rocha GA et al (2012) Microencapsulation of lycopene by gelatin-pectin complex coacervation. J Food Proc Preserv 36:185–190

    Article  CAS  Google Scholar 

  • Talavera S, Felgines C, Texier O et al (2003) Anthocyanins are efficiently absorbed from the stomach in anesthetized rats. J Nutr 133:4178–4182

    Article  CAS  Google Scholar 

  • Yang M, Koo SI, Song WO et al (2011) Food matrix affecting anthocyanin bioavailability: review. Curr Med Chem 18:291–300

    Article  CAS  Google Scholar 

  • Yao KD, Tu H, Cheng F et al (1997) pH-sensitivity of the swelling of a chitosan-pectin polyelectrolyte complex. Die Angewandte Makromolekulare Chemie 245:63–72

    Article  CAS  Google Scholar 

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Acknowledgments

Authors are grateful to Head, Division of Agricultural Chemicals for providing all the facilities. Authors are also thankful to ICAR for funding this work. The present work was funded by the Senior Research Fellowship, ICAR, New Delhi.

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Authors and Affiliations

  1. Division of Agricultural Chemicals, ICAR-Indian Agricultural Research Institute, New Delhi, 110 012, India

    Rohan Sarkar, Anirban Dutta & Supradip Saha

  2. Physics and Energy Harvesting, CSIR-National Physical Laboratory, New Delhi, India

    Asit Patra

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  1. Rohan Sarkar
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  2. Anirban Dutta
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  4. Supradip Saha
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Correspondence to Supradip Saha.

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Sarkar, R., Dutta, A., Patra, A. et al. Bio-inspired biopolymeric coacervation for entrapment and targeted release of anthocyanin. Cellulose 28, 377–388 (2021). https://doi.org/10.1007/s10570-020-03523-w

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  • DOI: https://doi.org/10.1007/s10570-020-03523-w

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