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Structural and functional characterization of bacterial cellulose from Enterobacter hormaechei subsp. steigerwaltii strain ZKE7

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Abstract

The present investigation reports, the bacterial cellulose (BC) a high-purity polymer produced from isolated strain ZKE7. BC production was optimized by Plackett–Burman and central composite designs yielding 18.5 g/l BC with a 4.5-fold enhancement. BC composites functionalized with antibiotics, BC-gelatin hydrogel, BC-Chitosan hydrogel and BC silver nanoparticle composites were developed and evaluated for water retention capability, moisture content and protein adsorption. The drug release behavior of the composites was consistent for controlled drug delivery. Composites were assessed for functional characteristic such as antimicrobial properties. BC composites functionalized with Neotericine exhibited antifungal activity against Candida albicans. Other composites showed pronounced antibacterial properties against Escherichia coli, Bacillus subtilis and Micrococcus luteus. Structural and thermal characterization of BC composites was carried out by FTIR, SEM with energy dispersive X-ray analysis, TGA and differential scanning calorimetry analysis. The results reveal high BC production with excellent properties that can be employed in biomedical field.

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Acknowledgments

The first author who is the corresponding author thank KLE Technological University, Hubballi for providing funding to carry out the present research work through Research Group (RG) projects.

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Authors and Affiliations

  1. Department of Biotechnology, KLE Technological University, Vidyanagar, Hubballi, Karnataka, 580031, India

    Zabin K. Bagewadi, Venessa Dsouza, Sanjay H. Deshpande, Uday M. Muddapur, Deepak A. Yaraguppi & Jayateerth S. Bhavikatti

  2. Department of Biochemistry, School of Applied Sciences, REVA University, Bangalore, 560064, India

    Sikandar I. Mulla & Vaddi Damodara Reddy

  3. ICMR-National Institute of Traditional Medicine, Belagavi, Karnataka, 590010, India

    Sanjay H. Deshpande

  4. School of Basic and Applied Sciences, Dayananda Sagar University, Bangalore, 560078, India

    Sunil S. More

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  1. Zabin K. Bagewadi
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  2. Venessa Dsouza
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  3. Sikandar I. Mulla
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Contributions

ZB and VD have designed the experimental work, executed all the experiments and carried out all the data analysis. ZB, VD, SIM, SD, UM, DY, VDR, JB and SM were involved in interpretation of data. ZB and VD have written the manuscript. All the authors have approved the contents of the manuscript.

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Correspondence to Zabin K. Bagewadi.

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Bagewadi, Z.K., Dsouza, V., Mulla, S.I. et al. Structural and functional characterization of bacterial cellulose from Enterobacter hormaechei subsp. steigerwaltii strain ZKE7. Cellulose 27, 9181–9199 (2020). https://doi.org/10.1007/s10570-020-03412-2

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