Abstract
Because of the high specific surface area, polyporous structure and ease of preparation, porous biochar from lignocellulosic biomass is popular for being used as support for enzyme immobilization. In this work, polyporous biochar combined with magnetic particle γ-Fe2O3 was prepared by calcination and then used as support for cellulase adsorption. The effects of calcination temperature and time on the properties of magnetic polyporous biochar were investigated and the optimum preparation condition was obtained. For the cellulase adsorption, the immobilization capacity for the magnetic support reached as high as 266 mg/g with a relative activity of 73.6% compared with that of free cellulase. The behavior of cellulase adsorption showed that an endothermic process occurred more easily at high temperatures, which resulted in a high adsorption amount.
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References
Abbaszadeh M, Hejazi P (2019) Metal affinity immobilization of cellulase on Fe3O4 nanoparticles with copper as ligand for biocatalytic applications. Food Chem 290:47–55. https://doi.org/10.1016/j.foodchem.2019.03.117
Ahmad M, Upamali A, Eun J et al (2014) Biochar as a sorbent for contaminant management in soil and water: a review. Chemosphere 99:19–33. https://doi.org/10.1016/j.chemosphere.2013.10.071
Ahmed IN, Chang R, Tsai WB (2017) Poly(acrylic acid) nanogel as a substrate for cellulase immobilization for hydrolysis of cellulose. Colloids Surf B 152:339–343. https://doi.org/10.1016/j.colsurfb.2017.01.040
Altinkaynak C, Tavlasoglu S, Özdemir N, Ocsoy I (2016) A new generation approach in enzyme immobilization: organic-inorganic hybrid nanoflowers with enhanced catalytic activity and stability. Enzyme Microb Technol 93–94:105–112. https://doi.org/10.1016/j.enzmictec.2016.06.011
Amaly N, Si Y, Chen Y et al (2018) Reusable anionic sulfonate functionalized nanofibrous membranes for cellulase enzyme adsorption and separation. Colloids Surf B 170:588–595. https://doi.org/10.1016/j.colsurfb.2018.06.019
Azargohar R, Dalai AK (2008) Steam and KOH activation of biochar: experimental and modeling studies. Microporous Mesoporous Mater 110:413–421. https://doi.org/10.1016/j.micromeso.2007.06.047
Bansal P, Hall M, Realff MJ et al (2009) Modeling cellulase kinetics on lignocellulosic substrates. Biotechnol Adv 27:833–848. https://doi.org/10.1016/j.biotechadv.2009.06.005
Barbosa O, Torres R, Ortiz C et al (2013) Heterofunctional supports in enzyme immobilization: from traditional immobilization protocols to opportunities in tuning enzyme properties. Biomacromol 14:2433–2462. https://doi.org/10.1021/bm400762h
Bhattacharyya MS, Hiwale P, Piras M et al (2010) Lysozyme Adsorption and Release from Ordered Mesoporous Materials. J Phys Chem C 114:19928–19934
Cha JS, Park SH, Jung S et al (2016) Production and Utilization of Biochar: a Review. J Ind Eng Chem 40:1–15. https://doi.org/10.1016/j.jiec.2016.06.002
Chander R, Deswal D, Sharma S et al (2016) Revisiting cellulase production and rede fi ning current strategies based on major challenges. Renew Sustain Energy Rev 55:249–272. https://doi.org/10.1016/j.rser.2015.10.132
Chen B, Qiu J, Mo H et al (2017a) Synthesis of mesoporous silica with different pore sizes for cellulase immobilization: pure physical adsorption. New J Chem 41:9338–9345. https://doi.org/10.1039/c7nj00441a
Chen J, Qiu J, Wang B et al (2017b) Manganese dioxide/biocarbon composites with superior performance in supercapacitors. J Electroanal Chem 791:159–166. https://doi.org/10.1016/j.jelechem.2017.03.025
Chen J, Qiu J, Wang B et al (2017c) Fe3O4/biocarbon composites with superior performance in supercapacitors. J Electroanal Chem 804:232–239. https://doi.org/10.1016/j.jelechem.2017.09.028
Ghose TK (1987) Measurement of cellulase activities. Pure Appl Chem. https://doi.org/10.1351/pac198759020257
Haghighi S, Hossein A, Tabatabaei M (2013) Lignocellulosic biomass to bioethanol, a comprehensive review with a focus on pretreatment. Renew Sustain Energy Rev 27:77–93. https://doi.org/10.1016/j.rser.2013.06.033
Hudson S, Cooney J, Magner E (2008) Proteins in mesoporous silicates. Angew chemie Int Ed 47:8582–8594. https://doi.org/10.1002/anie.200705238
Ince A, Bayramoglu G, Karagoz B et al (2012) A method for fabrication of polyaniline coated polymer microspheres and its application for cellulase immobilization. Chem Eng J 189–190:404–412. https://doi.org/10.1016/j.cej.2012.02.048
Jordan J, Kumar CSSR, Theegala C (2011) Preparation and characterization of cellulase-bound magnetite nanoparticles. J Mol Catal B Enzym 68:139–146. https://doi.org/10.1016/j.molcatb.2010.09.010
Kumar G, Mudhoo A, Sivagurunathan P, Nagarajan D (2016) Recent insights into the cell immobilization technology applied for dark fermentative hydrogen production. Bioresour Technol 219:725–737. https://doi.org/10.1016/j.biortech.2016.08.065
Lei J, Fan J, Yu C et al (2004) Immobilization of enzymes in mesoporous materials: controlling the entrance to nanospace. Microporous Mesoporous Mater 73:121–128. https://doi.org/10.1016/j.micromeso.2004.05.004
Lin Y, Liu X, Xing Z et al (2017) Preparation and characterization of magnetic Fe3O4—chitosan nanoparticles for cellulase immobilization. Cellulose 24:5541–5550. https://doi.org/10.1007/s10570-017-1520-6
Monte F, Morales MP, Levy D et al (1997) Formation of γ-Fe2O3 isolated nanoparticles in a silica matrix. Langmuir 7463:3627–3634
Piras M, Salis A, Piludu M, Monduzzi M (2011) 3D vision of human lysozyme adsorbed onto a SBA-15 nanostructured matrixw. Chem Commun 47:7338–7340. https://doi.org/10.1039/c1cc11840d
Qian K, Kumar A, Zhang H et al (2015) Recent advances in utilization of biochar. Renew Sustain Energy Rev 42:1055–1064. https://doi.org/10.1016/j.rser.2014.10.074
Ramakrishnan S, Krainer G, Grundmeier G et al (2016) Structural stability of DNA origami nanostructures in the presence of chaotropic agents. Nanoscale 8:10398–10405. https://doi.org/10.1039/c6nr00835f
Rusmini F, Zhong Z, Feijen J (2007) Protein immobilization strategies for protein biochips. Biomacromol 8:1775–1789
Sassner P, Galbe M, Zacchi G (2008) Techno-economic evaluation of bioethanol production from three different lignocellulosic materials. Biomass Bioenergy 32:422–430. https://doi.org/10.1016/j.biombioe.2007.10.014
Teofil J, Jakub Z, Krajewska B (2014) Enzyme immobilization by adsorption: a review. Adsorption. https://doi.org/10.1007/s10450-014-9623-y
Thompson KA, Shimabuku KK, Kearns JP et al (2016) Environmental comparison of biochar and activated carbon for tertiary wastewater treatment. Environ Sci Technol 50:11253–11262
Wu H, Wu G, Wang L (2015) Peculiar porous α-Fe2O3, γ-Fe2O3 and Fe3O4 nanospheres: facile synthesis and electromagnetic properties. Powder Technol 269:443–451. https://doi.org/10.1016/j.powtec.2014.09.045
Yuichi M, Shin-ichi K, Yuki K, Katsuya K (2014) Interparticle mesoporous silica as an effective support for enzyme immobilisation. RSC Adv 4:3573–3580. https://doi.org/10.1039/c3ra46122j
Zang L, Qiu J, Wu X et al (2014) Preparation of magnetic chitosan nanoparticles as support for cellulase immobilization. Ind Eng Chem Res 53:3448–3454. https://doi.org/10.1021/ie404072s
Zhou HM Z (2013) Progress in enzyme immobilization in ordered mesoporous materials and related applications. Chem Soc Rev 42:3894–3912. https://doi.org/10.1039/c3cs60059a
Acknowledgments
We thank associate Prof. Komiyama for the help of VEM test and Dr. Wang for the help of BET test in this study.
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Mo, H., Qiu, J., Yang, C. et al. Preparation and characterization of magnetic polyporous biochar for cellulase immobilization by physical adsorption. Cellulose 27, 4963–4973 (2020). https://doi.org/10.1007/s10570-020-03125-6
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DOI: https://doi.org/10.1007/s10570-020-03125-6