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Thermosensitive hydrogels based on methylcellulose derivatives for prevention of postoperative adhesion

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Abstract

Postoperative adhesions commonly cause severe complications, and also increase the risk of adhesiolysis. To improve postoperative adhesion, a series of 2-methacryloyloxy ethyl phosphorylcholine conjugated methylcellulose (MC-g-MPC) derivatives were synthesized to utilize the anti-protein adsorption of 2-methacryloyloxy ethyl phosphorylcholine and the thermosensitivity of methylcellulose. The structures of MC-g-MPC derivatives were characterized by Fourier transform infrared spectroscopy (FTIR) and 1H nuclear magnetic resonance (1H NMR) analyses, and their thermosensitivities were studied by rheological analysis. The gelling temperature of the MC-g-MPC hydrogel was 33 °C and gelling time of the hydrogel under body temperature was 2.5 min. The morphology of the MC-g-MPC hydrogel was observed by scanning electron microscopy (SEM). The results of in vitro cytotoxicity and immunotoxicity, and hemolysis assays demonstrated the good biocompatibility of MC-g-MPC hydrogels. Following the injection of the MC-g-MPC hydrogel, the postoperative adhesion of damaged ceca in Sprague–Dawley rats was improved when compared with a control group (no treatment). In addition, MC-g-MPC-0.25 hydrogel could downregulate the expression of Col-I and Col-IV collagen proteins, thus exert its anti-protein absorption capacity. Therefore, the MC-g-MPC hydrogel is a promising biomaterial for preventing postoperative adhesion.

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Acknowledgments

We acknowledge support from the Special Funds for Public Welfare Research and Capacity Building of Guangdong Province in China (2016A020222017 and 2016A010103014), the Key project of Guangdong Natural Science Foundation of China (2017B030311007), and the Science and Technology Planning Project of Guangdong Province in China (2012B091100356).

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  1. Yaqing Huang and Weihong Guo have contributed equally to this work.

Authors and Affiliations

  1. Department of Polymer and Materials Science, School of Chemistry, Key Laboratory for Polymeric Composite and Functional Materials of Ministry of Education, Guangdong Provincial Key Laboratory for High Performance Polymer-based Composites, Sun Yat-sen University, Guangzhou, 510275, China

    Yaqing Huang, Junzhao Zhang, Xiao Peng, Li-Ming Zhang & Liqun Yang

  2. Department of General Surgery, Nanfang Hospital, Guangdong Provincial Engineering Technology Research Center of Minimally Invasive Surgery, Southern Medical University, 1838 North Guangzhou Avenue, Guangzhou, 510515, China

    Weihong Guo & Guoxin Li

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  1. Yaqing Huang
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Huang, Y., Guo, W., Zhang, J. et al. Thermosensitive hydrogels based on methylcellulose derivatives for prevention of postoperative adhesion. Cellulose 27, 1555–1571 (2020). https://doi.org/10.1007/s10570-019-02857-4

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