, Volume 26, Issue 13–14, pp 7939–7952 | Cite as

Consumption of sugars and inhibitors of softwood hemicellulose hydrolysates as carbon sources for polyhydroxybutyrate (PHB) production with Paraburkholderia sacchari IPT 101

  • Karolin Dietrich
  • Marie-Josée DumontEmail author
  • Valérie Orsat
  • Luis F. Del Rio
Original Research


The future industrial success of the compostable bio-polyesters known as polyhydroxyalkanoates, which includes polyhydroxybutyrate (PHB), depends mainly on their production using cheaper carbon sources than food-derived glucose. The existing pulp and paper infrastructure enables an alternative sugar supply in the form of wood hydrolysates. Softwood hemicellulose hydrolysates have a favourable sugar profile for fermentations and can be produced in such emerging integrated forest biorefineries. The processes can lead to varying amounts of inhibitors which can lead to the reduction or prevention of bacterial growth. A dilute acid pretreatment was used to produce a softwood hemicellulose hydrolysate containing the sugars mannose, xylose, glucose, galactose, and arabinose. To study the effects of increasing sugar diversity and inhibitor concentration, the softwood hydrolysate was mixed in varying proportions with a hardwood holocellulose hydrolysate obtained from the TMP-Bio process (a thermomechanical pulping-based process producing glucose and xylose). In fermentations with Paraburkholderia sacchari IPT 101, the sugars were depleted at different rates in the following order: glucose, mannose, xylose, galactose, and arabinose. All potential inhibitors except phenols were metabolized. The maximum cell dry weight reached 6.7 ± 0.1 g/L with 71 ± 5% PHB with hardwood holocellulose hydrolysate after 48 h. The analyses of the sugar and inhibitor consumption provided valuable information to validate approaches for the detoxification of softwood hemicellulose hydrolysates. Overall, the detoxification would allow PHB production in an integrated softwood biorefinery scheme.


Softwood Hardwood Inhibitors PHA Biorefinery 



Analysis of variance


Cell dry mass


Hardwood holocellulose hydrolysate from thermomechanical pulping




Ion chromatography


Not determined


Optical density














Softwood hemicellulose hydrolysate from dilute acid hydrolysis


Volatile fatty acids



The financial support from the Richard H. Tomlinson Doctoral Fellowship and the National Science and Engineering Research Council of Canada (NSERC) is gratefully acknowledged.

Compliance of ethical standards

Conflict of interest

The authors declare that they have no competing interests.

Supplementary material

10570_2019_2664_MOESM1_ESM.pdf (669 kb)
Supplementary material 1 (PDF 668 kb)


  1. Bowers T, Vaidya A, Smith DA, Lloyd-Jones G (2014) Softwood hydrolysate as a carbon source for polyhydroxyalkanoate production. J Chem Technol Biotechnol 89:1030–1037. CrossRefGoogle Scholar
  2. Cavalheiro JM, Raposo RS, de Almeida MCMD, Cesário MT, Sevrin C, Grandfils C, da Fonseca MMR (2012) Effect of cultivation parameters on the production of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) and poly(3-hydroxybutyrate-4-hydroxybutyrate-3-hydroxyvalerate) by Cupriavidus necator using waste glycerol. Bioresour Technol 111:391–397. CrossRefPubMedGoogle Scholar
  3. Cesário MT, de Almeida MCMD (2015) Lignocellulosic hydrolysates for the production of polyhydroxyalkanoates. In: Kamm B (ed) Microorganisms in biorefineries. Springer, Berlin, pp 79–104. CrossRefGoogle Scholar
  4. Cesário MT, Raposo RS, de Almeida MCMD, van Keulen F, Ferreira BS, da Fonseca MMR (2014) Enhanced bioproduction of poly-3-hydroxybutyrate from wheat straw lignocellulosic hydrolysates. New Biotechnol 31:104–113. CrossRefGoogle Scholar
  5. Chen G-Q (2010) Industrial production of PHA. In: Chen GG-Q (ed) Plastics from bacteria: natural functions and applications. Microbiology monographs, vol 14. Springer, Berlin, pp 121–132. CrossRefGoogle Scholar
  6. Clements LD, Van Dyne DL (2008) The lignocellulosic biorefinery—a strategy for returning to a sustainable source of fuels and industrial organic chemicals. In: Kamm B, Gruber PR, Kamm M (eds) Biorefineries—industrial processes and products. Wiley, Weinheim, pp 115–128. CrossRefGoogle Scholar
  7. Dietrich K, Dumont M-J, Del Rio LF, Orsat V (2017) Producing PHAs in the bioeconomy towards a sustainable bioplastic. Sustain Prod Consum 9:58–70. CrossRefGoogle Scholar
  8. Dietrich K, Dumont M-J, Schwinghamer T, Orsat V, Del Rio LF (2018) Model study to assess softwood hemicellulose hydrolysates as the carbon source for PHB production in Paraburkholderia sacchari IPT 101. Biomacromolecules 19(1):188–200. CrossRefPubMedGoogle Scholar
  9. European Bioplastics (2018) Bioplastics market data. Accessed 26 June 2018
  10. Fatehi P, Ni Y (2011) Integrated forest biorefinery—prehydrolysis/dissolving pulping process. In: Zhu JY, Zhang X, Pan XJ (eds) Sustainable production of fuels, chemicals, and fibers from forest biomass. ACS symposium series. American Chemical Society, Washington, pp 475–506. CrossRefGoogle Scholar
  11. Geyer R, Jambeck JR, Law KL (2017) Production, use, and fate of all plastics ever made. Sci Adv 3:1–5. CrossRefGoogle Scholar
  12. Gírio FM, Fonseca C, Carvalheiro F, Duarte LC, Marques S, Bogel-Łukasik R (2010) Hemicelluloses for fuel ethanol: a review. Bioresour Technol 101:4775–4800. CrossRefPubMedGoogle Scholar
  13. Jönsson LJ, Martín C (2016) Pretreatment of lignocellulose: formation of inhibitory by-products and strategies for minimizing their effects. Bioresour Technol 199:103–112. CrossRefPubMedGoogle Scholar
  14. Kim BS, Lee SC, Lee SY, Chang HN, Chang YK, Woo SI (1994) Production of poly(3-hydroxybutyric acid) by fed-batch culture of Alcaligenes eutrophus with glucose concentration control. Biotechnol Bioeng 43:892–898. CrossRefPubMedGoogle Scholar
  15. Koller M, Dias MMD, Rodriguez-Contreras A, Kunaver M, Zagar E, Krzan A, Braunegg G (2015) Liquefied wood as inexpensive precursor-feedstock for bio-mediated incorporation of (R)-3-hydroxyvalerate into polyhydroxyalkanoates. Materials 8:6543–6557. CrossRefPubMedPubMedCentralGoogle Scholar
  16. Koller M, Maršálek L, de Sousa Dias MM, Braunegg G (2017) Producing microbial polyhydroxyalkanoate (PHA) biopolyesters in a sustainable manner. New Biotechnol 37(Part A):24–38. CrossRefGoogle Scholar
  17. Kucera D, Benesova P, Ladicky P, Pekar M, Sedlacek P, Obruca S (2017) Production of polyhydroxyalkanoates using hydrolyzates of spruce sawdust: comparison of hydrolyzates detoxification by application of overliming, active carbon, and lignite. Bioengineering 4:53. CrossRefPubMedPubMedCentralGoogle Scholar
  18. Kumar P, Barrett DM, Delwiche MJ, Stroeve P (2009) Methods for pretreatment of lignocellulosic biomass for efficient hydrolysis and biofuel production. Ind Eng Chem Res 48:3713–3729. CrossRefGoogle Scholar
  19. Lehninger AL, Nelson DL, Cox MM (2017) Lehninger principles of biochemistry, 7th edn. Freeman, New YorkGoogle Scholar
  20. Mao C, Del Rio LF, Wafa Al Dajani W, Wong D, Yuan Z, Browne T (2015) FPInnovations’ TMP-bio for lignocellulosic biomass: current state and scaling up. In: The 6th Nordic wood biorefinery conference, Helsinki, FinlandGoogle Scholar
  21. Raposo RS, de Almeida MCMD, de Oliveira MdCMA, da Fonseca MM, Cesário MT (2017) A Burkholderia sacchari cell factory: production of poly-3-hydroxybutyrate, xylitol and xylonic acid from xylose-rich sugar mixtures. New Biotechnol 34:12–22. CrossRefGoogle Scholar
  22. Silva JA, Tobella LM, Becerra J, Godoy F, Martínez MA (2007) Biosynthesis of poly-β-hydroxyalkanoate by Brevundimonas vesicularis LMG P-23615 and Sphingopyxis macrogoltabida LMG 17324 using acid-hydrolyzed sawdust as carbon source. J Biosci Bioeng 103:542–546. CrossRefPubMedGoogle Scholar
  23. Singleton VL, Orthofer R, Lamuela-Raventós RM (1999) Analysis of total phenols and other oxidation substrates and antioxidants by means of folin-ciocalteu reagent. In: Packer L (ed) Methods in enzymology, vol 299. Elsevier, Amsterdam, pp 152–178Google Scholar
  24. Sjöström E, Alén R (1999) Analytical methods in wood chemistry, pulping, and papermaking. Springer, BerlinCrossRefGoogle Scholar
  25. Wafa Al Dajani W, Mao C, Yuan Z, Browne T (2013) Fermentation of TMP-bio process sugar hydrolysate to produce lactic acid. FPInnovations PRR 1959Google Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Bioresource Engineering DepartmentMcGill UniversitySte-Anne de BellevueCanada
  2. 2.FPInnovationsPointe-ClaireCanada

Personalised recommendations