Enzymatic production of cello-oligomers with endoglucanases

Kluge, Stefanie; Bonhage, Benjamin; Viell, Jörn; Granström, Mari; Kindler, Alois; Spiess, Antje C.

doi:10.1007/s10570-019-02390-4

Original Research
Published: 27 March 2019

Enzymatic production of cello-oligomers with endoglucanases

Stefanie Kluge^1,2,
Benjamin Bonhage¹,
Jörn Viell³,
Mari Granström⁴,
Alois Kindler⁴ &
Antje C. Spiess^1,2,5

Cellulose volume 26, pages 4279–4290 (2019)Cite this article

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Abstract

Cello-oligomers have a wide application range and are gained from three different cello-oligomer production paths, which show different drawbacks, namely acid or alkaline hydrolysis of cellulose, chemical or enzymatic synthesis, and enzymatic hydrolysis of cellulose using cellulase mixtures. Therefore, this study aims at a novel route towards cello-oligomers using purified endoglucanases. From the renewable raw material cellulose, pretreated by an ionic liquid, cello-oligomers with a weight average degree of polymerization (DP_W) of 65 were directly obtained by enzymatic hydrolysis. During hydrolysis, between 14.1 and 24.5 mass percent of monomeric and dimeric sugars were formed as byproduct. A second ionic liquid pretreatment of the cellulose, remaining after the first hydrolysis, and a second enzymatic hydrolysis resulted in cello-oligomers with a DP of 35. XRD deconvolution and Raman analysis confirmed that crystallinity remained unchanged during enzymatic cellulose hydrolysis.

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Abbreviations

AgrBG:: Beta-glucosidases from Agrobacterium sp
AnBG:: Beta-glucosidases from A. niger
AnEG:: Endoglucanase from A. niger
BaEG:: Endoglucanase from B. amyloliquefaciens
BG:: Beta-glucosidases
CBH:: Cellobiohydrolases
CrI:: Crystallinity index
DP_W :: Weight average degree of polymerization
EG:: Endoglucanase
[EMIM] [Ac]:: 1-Ethyl-3-methylimidazolium acetate
GPC:: Gel permeation chromatography
IL:: Ionic liquid
MALLS:: Multiangle laser light scattering
RI:: Refractive index
TeEG:: Endoglucanase from T. emersonii
TmEG:: Endoglucanase from T. maritima
TlEG:: Endoglucanase from T. longibrachiatum
XRD:: X-ray diffraction

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Acknowledgments

Parts of this work were performed as part of the Cluster of Excellence “Tailor-Made Fuels from Biomass”, which is funded by the Excellence Initiative by the German federal and state governments to promote science and research at German universities.

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Authors and Affiliations

AVT - Enzyme Process Technology, RWTH Aachen University, Aachen, Germany
Stefanie Kluge, Benjamin Bonhage & Antje C. Spiess
DWI – Leibniz Institute for Interactive Materials, Aachen, Germany
Stefanie Kluge & Antje C. Spiess
AVT - Process Systems Engineering, RWTH Aachen University, Aachen, Germany
Jörn Viell
BASF SE, Ludwigshafen, Germany
Mari Granström & Alois Kindler
Institute for Biochemical Engineering, TU Braunschweig, Rebenring 56, 38106, Braunschweig, Germany
Antje C. Spiess

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Stefanie Kluge
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Benjamin Bonhage
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Jörn Viell
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Mari Granström
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Alois Kindler
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Antje C. Spiess
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Correspondence to Antje C. Spiess.

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Kluge, S., Bonhage, B., Viell, J. et al. Enzymatic production of cello-oligomers with endoglucanases. Cellulose 26, 4279–4290 (2019). https://doi.org/10.1007/s10570-019-02390-4

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Received: 26 June 2018
Accepted: 22 March 2019
Published: 27 March 2019
Issue Date: 15 May 2019
DOI: https://doi.org/10.1007/s10570-019-02390-4

Keywords

Biocatalysis
Biomaterials
Biopolymers
Cello-oligomers
Endoglucanases
Ionic liquid