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Cellulose

, Volume 25, Issue 7, pp 4179–4192 | Cite as

Biobleaching of waste paper using lignolytic enzyme from Fusarium equiseti VKF2: a mangrove isolate

  • Vinod Kumar Nathan
  • Subha Rajam Kanthimathinathan
  • Mary Esther Rani
  • Gunaseeli Rathinasamy
  • N. D. Kannan
Original Paper
  • 53 Downloads

Abstract

Laccase is a major enzyme used in the paper and pulp industries for bio-bleaching applications. This paper focused on the bio-bleaching property of a potential lignolytic fungus, Fusarium equiseti VKF-2. Optimum conditions for laccase production were tested by statistical models under solid state fermentation using saw dust as substrate. Fusarium equiseti VKF-2 achieved a maximum laccase activity of 305 Ug−1 of substrate. During purification, 67% yield was obtained with 80% ammonium sulphate. The enzyme activity was confirmed through zymogram analysis with 2 active fractions observed at 94 and 75 kDa. Laccase treatment resulted in defibrillations, crack formation and changes in the functional groups of paper pulp. The laccase facilitated the maximum hexenuronic acid release with Kappa number reduction. There was a Δ brightness of 15% following 4 h of enzymatic treatment. Hence, the present laccase was found to be suitable for the bio-bleaching of newspaper waste via an eco-friendly process.

Graphical abstract

Keywords

Laccase Fusarium equiseti Deinking Brightness Optimization 

Notes

Acknowledgments

Authors are thankful to Department of Science and Technology, Government of India and TNSCST for providing the grant to facilitate the research [Sanction Order No. DST/SSTP/TN/2K 10/126(G) 13-09-2011]. NVK thank Science and Engineering Research Board, Government of India for National Post Doctorate Fellowship [PDF/2016/00438].

Supplementary material

10570_2018_1834_MOESM1_ESM.docx (909 kb)
Supplementary material 1 (DOCX 909 kb)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Vinod Kumar Nathan
    • 1
    • 2
  • Subha Rajam Kanthimathinathan
    • 2
  • Mary Esther Rani
    • 2
  • Gunaseeli Rathinasamy
    • 2
  • N. D. Kannan
    • 3
  1. 1.Regional CentreNational Institute of Oceanography-CSIRCochinIndia
  2. 2.Department of Botany and MicrobiologyLady Doak CollegeMaduraiIndia
  3. 3.Department Plant Biotechnology, School of BiotechnologyMadurai Kamaraj UniversityMaduraiIndia

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