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Biobleaching of waste paper using lignolytic enzyme from Fusarium equiseti VKF2: a mangrove isolate

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Abstract

Laccase is a major enzyme used in the paper and pulp industries for bio-bleaching applications. This paper focused on the bio-bleaching property of a potential lignolytic fungus, Fusarium equiseti VKF-2. Optimum conditions for laccase production were tested by statistical models under solid state fermentation using saw dust as substrate. Fusarium equiseti VKF-2 achieved a maximum laccase activity of 305 Ug−1 of substrate. During purification, 67% yield was obtained with 80% ammonium sulphate. The enzyme activity was confirmed through zymogram analysis with 2 active fractions observed at 94 and 75 kDa. Laccase treatment resulted in defibrillations, crack formation and changes in the functional groups of paper pulp. The laccase facilitated the maximum hexenuronic acid release with Kappa number reduction. There was a Δ brightness of 15% following 4 h of enzymatic treatment. Hence, the present laccase was found to be suitable for the bio-bleaching of newspaper waste via an eco-friendly process.

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Acknowledgments

Authors are thankful to Department of Science and Technology, Government of India and TNSCST for providing the grant to facilitate the research [Sanction Order No. DST/SSTP/TN/2K 10/126(G) 13-09-2011]. NVK thank Science and Engineering Research Board, Government of India for National Post Doctorate Fellowship [PDF/2016/00438].

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Authors and Affiliations

  1. Regional Centre, National Institute of Oceanography-CSIR, Cochin, Kerala, 682018, India

    Vinod Kumar Nathan

  2. Department of Botany and Microbiology, Lady Doak College, Madurai, Tamil Nadu, 625 002, India

    Vinod Kumar Nathan, Subha Rajam Kanthimathinathan, Mary Esther Rani & Gunaseeli Rathinasamy

  3. Department Plant Biotechnology, School of Biotechnology, Madurai Kamaraj University, Madurai, 625021, India

    N. D. Kannan

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  1. Vinod Kumar Nathan
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  2. Subha Rajam Kanthimathinathan
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  3. Mary Esther Rani
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  5. N. D. Kannan
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Correspondence to Vinod Kumar Nathan.

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Nathan, V.K., Kanthimathinathan, S.R., Rani, M.E. et al. Biobleaching of waste paper using lignolytic enzyme from Fusarium equiseti VKF2: a mangrove isolate. Cellulose 25, 4179–4192 (2018). https://doi.org/10.1007/s10570-018-1834-z

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