Photocrosslinked methacrylated chitosan-based nanofibrous scaffolds as potential skin substitute
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Nanofibers based on natural polymers have recently been attracting research interest as promising materials for use as skin substitutes. Here, we prepared photocrosslinked nanofibrous scaffolds based on methacrylated chitosan (MACS) by photocrosslinking electrospun methacrylated chitosan/poly (vinyl alcohol) (PVA) mats and subsequently removing PVA from the nanofibers. We comprehensively investigated the solution properties of MACS/PVA precursors, the intermolecular action between MACS and PVA components, and the morphology of MACS/PVA nanofibers. Results indicated that the fiber diameter and morphology of the photocrosslinked methacrylated chitosan-based nanofibrous scaffolds were controlled by the MACS/PVA mass ratio and showed highly micro-porous structures with many fibrils. In vitro cytotoxicity evaluation and cell culture experiments confirmed that MACS-based mats with micro-pore structure were biocompatible with L929 cells and facilitated cellular migration into the 3D matrix, demonstrating their potential application as skin replacements for wound repair.
KeywordsElectrospinning Methacrylated chitosan Photopolymerization Skin substitute
This study was supported by National Natural Science Foundation of China (Grant Nos. 51203123, 51403165, 51503161).
- Jannesari M, Varshosaz J, Morshed M, Zamani M (2011) Composite poly (vinyl alcohol)/poly (vinyl acetate) electrospun nanofibrous mats as novel wound dressing matrix for controlled release of drugs. Int J Nanomed 6:993–1003Google Scholar