, Volume 24, Issue 10, pp 4253–4262 | Cite as

Photocrosslinked methacrylated chitosan-based nanofibrous scaffolds as potential skin substitute

  • Yingshan ZhouEmail author
  • Kaili Liang
  • Can Zhang
  • Jun Li
  • Hongjun Yang
  • Xin Liu
  • Xianze Yin
  • Dongzhi Chen
  • Weilin Xu
  • Pu XiaoEmail author
Original Paper


Nanofibers based on natural polymers have recently been attracting research interest as promising materials for use as skin substitutes. Here, we prepared photocrosslinked nanofibrous scaffolds based on methacrylated chitosan (MACS) by photocrosslinking electrospun methacrylated chitosan/poly (vinyl alcohol) (PVA) mats and subsequently removing PVA from the nanofibers. We comprehensively investigated the solution properties of MACS/PVA precursors, the intermolecular action between MACS and PVA components, and the morphology of MACS/PVA nanofibers. Results indicated that the fiber diameter and morphology of the photocrosslinked methacrylated chitosan-based nanofibrous scaffolds were controlled by the MACS/PVA mass ratio and showed highly micro-porous structures with many fibrils. In vitro cytotoxicity evaluation and cell culture experiments confirmed that MACS-based mats with micro-pore structure were biocompatible with L929 cells and facilitated cellular migration into the 3D matrix, demonstrating their potential application as skin replacements for wound repair.


Electrospinning Methacrylated chitosan Photopolymerization Skin substitute 



This study was supported by National Natural Science Foundation of China (Grant Nos. 51203123, 51403165, 51503161).

Supplementary material

10570_2017_1433_MOESM1_ESM.docx (516 kb)
Supplementary material 1 (DOCX 516 kb)


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Copyright information

© Springer Science+Business Media B.V. 2017

Authors and Affiliations

  • Yingshan Zhou
    • 1
    Email author
  • Kaili Liang
    • 1
  • Can Zhang
    • 1
  • Jun Li
    • 1
  • Hongjun Yang
    • 1
  • Xin Liu
    • 1
  • Xianze Yin
    • 1
  • Dongzhi Chen
    • 1
  • Weilin Xu
    • 1
  • Pu Xiao
    • 2
    Email author
  1. 1.College of Materials Science and EngineeringWuhan Textile UniversityWuhanPeople’s Republic of China
  2. 2.Centre for Advanced Macromolecular Design, School of ChemistryUniversity of New South WalesSydneyAustralia

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