Hemicellulose-reinforced nanocellulose hydrogels for wound healing application
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Polysaccharides are finding an increasing number of applications in medical and pharmaceutical fields thanks to their biodegradability, biocompatibility, and in some cases bioactivity. Two approaches were applied to use hemicelluloses as crosslinkers to tune the structural and mechanical properties of nanofibrillated cellulose (NFC) hydrogel scaffolds, and thus to investigate the effect of these properties on the cellular behavior during wound healing application. Different types of hemicellulose (galactoglucomannan (GGM), xyloglucan (XG), and xylan) were introduced into the NFC network via pre-sorption (Method I) and in situ adsorption (Method II) to reinforce the NFC hydrogels. The charge density of the NFC, the incorporated hemicellulose type and amount, and the swelling time of the hydrogels were found to affect the pore structure, the mechanical strength, and thus the cells’ growth on the composite hydrogel scaffolds. The XG showed the highest adsorption capacity on the NFC, the highest reinforcement effect, and facilitated/promoted cell growth. The pre-sorbed XG in the low-charged NFC network with a lower weight ratio (NFC/XG-90:10) showed the highest efficacy in supporting the growth and proliferation of fibroblast cells (NIH 3T3). These all-polysaccharide composite hydrogels may work as promising scaffolds in wound healing applications to provide supporting networks and to promote cells adhesion, growth, and proliferation.
KeywordsAll-polysaccharide composites Cell behavior Hemicelluloses Hydrogel Mechanical strength Nanofibrillated cellulose Reinforcing Wound healing
Jun Liu would like to acknowledge the financial support of the China Scholarship Council and Graduate School of Chemical Engineering of Åbo Akademi University. This work is also part of the activities at the Johan Gadolin Process Chemistry Centre, a Centre of Excellence appointed by Åbo Akademi University. NordForsk via the Refining Lignocellulosics to Advanced Polymers and Fibers (PolyRefNorth) network and the NORCEL project (Grant No. 228147) and NanoHeal project (Grant No. 219733), funded by the Research Council of Norway through the NANO2021 Program, are thanked for supporting the research exchange of Jun Liu at PFI. The Research Council of Norway is also acknowledged for the support to the Norwegian Micro- and Nano-Fabrication Facility, NorFab (Grant No. 197411/V30), which facilitated the AFM analysis. Ingebjorg Leirset, Per Olav Johnsen, Anne Reitan, Mirjana Filipovic, Storker Mor, and all other colleagues at PFI are acknowledged for assistance of the laboratory work.
- Andrijevic L, Radotic K, Bogdanovic J, Mutavdzic D, Bogdanovic G (2008) Antiproliferative effect of synthetic lignin against human breast cancer and normal fetal lung cell lines. Potency of low molecular weight fractions. J BUON 13:241–244Google Scholar
- Bodin A, Ahrenstedt L, Fink H, Brumer H, Risberg B, Gatenholm P (2007) Modification of nanocellulose with a xyloglucan-RGD conjugate enhances adhesion and proliferation of endothelial cells: implications for tissue engineering. Biomacromolecules 8:3697–3704. doi: 10.1021/bm070343q CrossRefGoogle Scholar
- Chang H-I, Wang Y (2011) Cell responses to surface and architecture of tissue engineering scaffolds. In: Daniel E (ed) Regenerative medicine and tissue engineering—cells and biomaterials. InTech, Croatla. doi: 10.5772/21983
- Malinen MM, Kanninen LK, Corlu A, Isoniemi HM, Lou Y-R, Yliperttula ML, Urtti AO (2014) Differentiation of liver progenitor cell line to functional organotypic cultures in 3D nanofibrillar cellulose and hyaluronan-gelatin hydrogels. Biomaterials 35:5110–5121. doi: 10.1016/j.biomaterials.2014.03.020 CrossRefGoogle Scholar
- Pereira MM et al (2013) Cytotoxicity and expression of genes involved in the cellular stress response and apoptosis in mammalian fibroblast exposed to cotton cellulose nanofibers. Nanotechnology 24Google Scholar
- Popa V (2011) Polysaccharides in medicinal and pharmaceutical applications. Smithers Rapra, ShrewsburyGoogle Scholar
- Portal O, Clark WA, Levinson DJ (2009) Microbial cellulose wound dressing in the treatment of nonhealing lower extremity ulcers. Wounds 21:1–3Google Scholar
- Richards RG (1996) The effect of surface roughness on fibroblast adhesion in vitro. Injury 27(Suppl 3):SC38–43Google Scholar
- Rosario P (2013) Advances in biomaterials science and biomedical applications. InTech. doi: 10.5772/56420
- Syverud K, Pettersen S, Draget K, Chinga-Carrasco G (2014) Controlling the elastic modulus of cellulose nanofibril hydrogels—scaffolds with potential in tissue engineering. Cellulose 1–9 doi: 10.1007/s10570-014-0470-5
- Willför S, Rehn P, Sundberg A, Sundberg K, Holmbom B (2003) Recovery of water-soluble acetylgalactoglucomannans from mechanical pulp of spruce. TAPPI J 2:27–32Google Scholar