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Development of probiotic tablet using alginate, pectin, and cellulose nanocrystals as excipients

Cellulose volume 23, pages 1967–1978 (2016)Cite this article

Abstract

The objective of this study is to develop a probiotic tablet formulation based on alginate, pectin, and cellulose nanocrystals (CNC) that can preserve high viability of a probiotic bacterium, Lactobacillus rhamnosus ATCC 9595, in gastrointestinal media as well as during storage. A central composite design including three independent biopolymeric material factors (alginate, pectin, and CNC) was applied, and 18 tablet formulations containing probiotic cells were formed. The optimized probiotic tablet based on alginate, pectin, and CNC showed 84 % cell viability after sequential transition through gastrointestinal media, whereas probiotic tablets prepared with alginate, pectin, and CNC individually showed only 19, 17, and 10 % probiotic viability, respectively. Swelling study also revealed that the optimized tablet underwent less dissolution in gastric solution (pH 1.5) and continuous dissolution in intestinal solution (pH 7). The optimized probiotic tablet was stored at 25 and 4 °C for up to 42 days, revealing a reduction of only 0.4 and 0.2 log colony forming units (CFU)/tablet, respectively, after 42 days.

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Acknowledgments

This research was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC) and by FPInnovations (Pointe-Claire, Québec, Canada) through the RDC program. Tanzina Huq is the recipient of a scholarship from Universitaire Fondation Armand-Frappier.

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Authors and Affiliations

  1. Research Laboratories in Sciences Applied to Food, Canadian Irradiation Centre (CIC), Institute of Nutraceutical and Functional Foods, INRS-Institut Armand-Frappier, University of Quebec, 531 Boulevard des Prairies, Laval, QC, H7V 1B7, Canada

    Tanzina Huq, Khanh Dang Vu & Monique Lacroix

  2. Département des sciences du bois et de la forêt, Faculté de foresterie, géographie et géomatique, Université Laval, Laval, QC, G1V 0A6, Canada

    Bernard Riedl

  3. FPInnovations, 570 Boulevard St. Jean, Pointe-Claire, QC, H9R 3J9, Canada

    Jean Bouchard

  4. Department of Food Bioscience and Technology, College of Life Sciences and Biotechnology, Korea University, Seoul, 136-701, Korea

    Jaejoon Han

Authors
  1. Tanzina Huq
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  2. Khanh Dang Vu
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  3. Bernard Riedl
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  4. Jean Bouchard
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  5. Jaejoon Han
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  6. Monique Lacroix
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Correspondence to Monique Lacroix.

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Huq, T., Vu, K.D., Riedl, B. et al. Development of probiotic tablet using alginate, pectin, and cellulose nanocrystals as excipients. Cellulose 23, 1967–1978 (2016). https://doi.org/10.1007/s10570-016-0905-2

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  • DOI: https://doi.org/10.1007/s10570-016-0905-2

Keywords

  • Cellulose nanocrystals
  • Pectin
  • Alginate
  • Probiotic tablet
  • Central composite design