, Volume 23, Issue 3, pp 1967–1978 | Cite as

Development of probiotic tablet using alginate, pectin, and cellulose nanocrystals as excipients

  • Tanzina Huq
  • Khanh Dang Vu
  • Bernard Riedl
  • Jean Bouchard
  • Jaejoon Han
  • Monique LacroixEmail author
Original Paper


The objective of this study is to develop a probiotic tablet formulation based on alginate, pectin, and cellulose nanocrystals (CNC) that can preserve high viability of a probiotic bacterium, Lactobacillus rhamnosus ATCC 9595, in gastrointestinal media as well as during storage. A central composite design including three independent biopolymeric material factors (alginate, pectin, and CNC) was applied, and 18 tablet formulations containing probiotic cells were formed. The optimized probiotic tablet based on alginate, pectin, and CNC showed 84 % cell viability after sequential transition through gastrointestinal media, whereas probiotic tablets prepared with alginate, pectin, and CNC individually showed only 19, 17, and 10 % probiotic viability, respectively. Swelling study also revealed that the optimized tablet underwent less dissolution in gastric solution (pH 1.5) and continuous dissolution in intestinal solution (pH 7). The optimized probiotic tablet was stored at 25 and 4 °C for up to 42 days, revealing a reduction of only 0.4 and 0.2 log colony forming units (CFU)/tablet, respectively, after 42 days.


Cellulose nanocrystals Pectin Alginate Probiotic tablet Central composite design 



This research was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC) and by FPInnovations (Pointe-Claire, Québec, Canada) through the RDC program. Tanzina Huq is the recipient of a scholarship from Universitaire Fondation Armand-Frappier.


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Copyright information

© Her Majesty the Queen in Right of Canada 2016

Authors and Affiliations

  • Tanzina Huq
    • 1
  • Khanh Dang Vu
    • 1
  • Bernard Riedl
    • 2
  • Jean Bouchard
    • 3
  • Jaejoon Han
    • 4
  • Monique Lacroix
    • 1
    Email author
  1. 1.Research Laboratories in Sciences Applied to Food, Canadian Irradiation Centre (CIC), Institute of Nutraceutical and Functional Foods, INRS-Institut Armand-FrappierUniversity of QuebecLavalCanada
  2. 2.Département des sciences du bois et de la forêt, Faculté de foresterie, géographie et géomatiqueUniversité LavalLavalCanada
  3. 3.FPInnovationsPointe-ClaireCanada
  4. 4.Department of Food Bioscience and Technology, College of Life Sciences and BiotechnologyKorea UniversitySeoulKorea

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