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Conferring antioxidant capacity to cellulose based materials by using enzymatically-modified products

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Abstract

A new, industrially feasible method for conferring cellulosic substrates antioxidant properties by using enzymatic products was developed. The method allows cellulose surfaces such as those of paper sheets to be conferred antioxidant capacity by using a functionalization solution (FS) obtained from an enzymatic reaction. Various laccases and different phenolic compounds (PhCs) potentially possessing antioxidant action were used to prepare the FS. Antioxidant capacity was assessed by using UV spectrophotometry to monitor the inhibition of ABTS radical cation (ABTS·+) in the presence of antioxidants. Based on the results, enzymatic modification of the phenolic compound in the FS increases the bonding strength of its components to cellulosic materials. Evidences on the grafting of the FS onto the cellulosic sheets, and changes in the chemical structure of the resulting oxidized form of PhCs were assessed by using ATR–FTIR. The level of antioxidant capacity achieved depends on the enzyme type, the chemical structure of the compounds in FS, and the presence of lignosulfonates in it. The potential of the proposed method for conferring antioxidant properties to cellulose-based materials by surface application of a product obtained from an enzymatic reaction is demonstrated here for the first time.

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Abbreviations

ABTS:

2,2′-Azino-bis(3-ethylbenzothiazoline-6-sulphonic acid)

Fb:

Cerrena unicolor enzyme supplied by Fungal Bioproducts®

FS:

Functionalization solution containing Lacc, PhC, and SL

FSU:

Functionalization solution uptake

KFS:

Control FS containing one or two of the additives Lacc, LG, SL in various combinations

Lacc:

Laccase

LG:

Lauryl gallate, dodecyl 3,4,5-trihydroxybenzoate

Mt:

Myceliophthora thermophila enzyme

PhC:

Phenolic compound

PS:

Paper sheets

SL:

Sulfonated lignin

TEAC:

Trolox equivalent antioxidant capacity

Tv:

Trametes villosa enzyme

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Acknowledgments

The authors are especially grateful to the Integrated Project of the Sixth Framework Program BIORENEW (NMP2-CT-2006-026456). The authors also thank the “Ministerio de Economía y Competitividad” of Spain for their support in this work under the projects BIOSURFACEL CTQ2012-34109 (funding also from the “Fondo Europeo de Desarrollo Regional” FEDER) and BIOPAPµFLUID (CTQ2013-48995-C2-1-R). Special thanks are also due to the consolidated research group AGAUR 2014 SGR 534 at Universitat de Barcelona (UB).

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Authors and Affiliations

  1. CELBIOTECH_Paper Engineering Research Group, Universitat Politècnica de Catalunya. BarcelonaTech, Colom 11, 08222, Terrassa, Spain

    Oriol Cusola, Cristina Valls, Teresa Vidal & M. Blanca Roncero

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  1. Oriol Cusola
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  2. Cristina Valls
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  3. Teresa Vidal
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  4. M. Blanca Roncero
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Correspondence to M. Blanca Roncero.

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Cusola, O., Valls, C., Vidal, T. et al. Conferring antioxidant capacity to cellulose based materials by using enzymatically-modified products. Cellulose 22, 2375–2390 (2015). https://doi.org/10.1007/s10570-015-0668-1

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  • DOI: https://doi.org/10.1007/s10570-015-0668-1

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