Evidence for differential interaction mechanism of plant cell wall matrix polysaccharides in hierarchically-structured bacterial cellulose
- 714 Downloads
The interaction mechanism of two plant cell wall polysaccharides, arabinoxylan and xyloglucan, with cellulose has been investigated by means of bacterial cellulose fermentation to mimic the cell wall biosynthesis process. The combination of small angle scattering techniques with XRD and SEM has enabled the identification of different structural features comprising hierarchically-assembled bacterial cellulose, i.e. cellulose microfibrils and ribbons. The SANS results have been described by a core–shell formalism, which accounts for the presence of regions with different solvent accessibility and supports the existence of microfibril sub-structure within the ribbons. Additionally, SAXS and XRD results suggest that the microfibril packing and crystalline structure are not affected by arabinoxylan, while xyloglucan interferes with the crystallization and assembly processes, resulting in less crystalline Iβ-rich microfibrils. This specific interaction mechanism is therefore crucial for the cellulose microfibril cross-linking effect of xyloglucan in plant cell walls. It is proposed that the distinct interaction mechanisms identified have their origin in the differential structural role of arabinoxylan and xyloglucan in plant cell walls.
KeywordsSmall angle scattering Neutron scattering X-ray scattering Cellulose Plant cell wall
The authors thank Dr. Joel Davis (ANSTO, Institute of Materials Engineering) for performing the SEM imaging and Dr. Tracey Hanley (ANSTO, Institute of Materials Engineering) for her valuable assistance with the XRD experiments. Dongjie Wang is acknowledged for composites production and SEM of fresh samples. M.M.S. would like to acknowledge a postdoctoral fellowship jointly funded by ANSTO and the ARC Centre of Excellence in Plant Cell Walls.
- Abràmoff MD, Magalhães PJ, Ram SJ (2004) Image processing with imageJ. Biophotonics Int 11:36–41Google Scholar
- Fan L, Degen M, Bendle S, Grupido N, Ilavsky J (2010) The absolute calibration of a small-angle scattering instrument with a laboratory X-ray source. J Phys Conf Ser. doi: 10.1088/1742-6596/247/1/012005
- Fink HP, Hofmann D, Philipp B (1995) Some aspects of lateral chain order in cellulosics from X-ray scattering. Cellulose 2:51–70Google Scholar
- Gilbert EP, Lopez-Rubio A, Gidley MJ (2012) Characterisation techniques in food materials science. In: Bhesh B, Yrjo R (eds) Food materials science and engineering. Wiley-Blackwell, p 52–93Google Scholar
- Mikkelsen D, Flanagan BM, Wilson SM, Bacic A, Gidley MJ (2015) Bacterial cellulose composites suggest arabinoxylans and (1 → 3)(1 → 4)-β-d-glucans are not functional equivalents of either xyloglucans or pectins in plant cell walls. Biomacromolecules (in press)Google Scholar