The present study was undertaken to develop edible nisin-microencapsulated beads in order to inhibit growth of Listeria monocytogenes in ready-to-eat (RTE) ham. Different concentrations of nisin (16, 31, and 63 μg/ml) were microencapsulated into alginate-cellulose nanocrystal beads. Microencapsulation kept the available nisin (63 μg/ml) content 20 times greater compared with free nisin (63 μg/ml) during 28 days of storage at 4 °C. Results showed that 63 μg/ml microencapsulated nisin exhibited 31.26 μg/ml available nisin content after 28 days of storage at 4 °C, whereas there was no available nisin content left for free nisin. Cooked ham slices were then coated by the microencapsulated nisin beads, inoculated with L. monocytogenes [~3 log colony-forming units (CFU)/g], and stored at 4 °C under vacuum packaging for 28 days. The beads containing 16, 31, and 63 μg/ml nisin significantly (P ≤ 0.05) reduced the L. monocytogenes counts by 2.65, 1.50, and 3.04 log CFU/g after 28 days of storage compared with free nisin. Furthermore, microencapsulated nisin beads did not change the physicochemical properties (pH and color) of RTE ham during storage.
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This research was supported by the Natural Sciences and Engineering Research Council of Canada (NSERC) and by FPInnovations (Pointe-Claire, Quebec, Canada) through the RDC program. The authors would also like to thank BSA Food Ingredients s.e.c./l.p. for providing salt ingredients (Montreal, Quebec, Canada). Tanzina Huq is the recipient of a scholarship from Fondation Universitaire Armand-Frappier.
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Huq, T., Riedl, B., Bouchard, J. et al. Microencapsulation of nisin in alginate-cellulose nanocrystal (CNC) microbeads for prolonged efficacy against Listeria monocytogenes . Cellulose 21, 4309–4321 (2014). https://doi.org/10.1007/s10570-014-0432-y
- Listeria monocytogenes
- RTE meat
- Cellulose nanocrystals