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Enhancing enzymatic hydrolysis of crystalline cellulose and lignocellulose by adding long-chain fatty alcohols

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Abstract

The effects of long-chain fatty alcohols (LFAs) on the enzymatic hydrolysis of crystalline cellulose by two commercial Trichoderma reesei cellulase cocktails (CTec2 and Celluclast 1.5L) were studied. It was found that n-butanol inhibited the enzymatic hydrolysis, but n-octanol, n-decanol and n-dodecanol had strong enhancement on enzymatic hydrolysis of crystalline cellulose in the buffer pH range from 4.0 to 6.0. LFAs can increase the hydrolysis efficiency of crystalline cellulose from 37 to 57 % at Celluclast 1.5L loading of ten filter paper units (FPU)/g glucan. LFAs have similar enhancement on the enzymatic hydrolysis of crystalline cellulose mixed with lignin or xylan. The enhancement of LFAs increased with the decrease of the crystallinity index. LFAs not only enhanced the high-solid enzymatic hydrolysis of lignocellulose, but also improved the rheological properties of high-solid lignocellulosic slurries by decreasing the yield stress and complex viscosity. Meanwhile, LFAs can improve the enzymatic hydrolysis of cellobiose to glucose, especially at low cellulase loading.

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Abbreviations

LFAs:

Long-chain fatty alcohols

CTec2:

Commercial Trichoderma reesei cellulase cocktail

FPU:

Filter paper units

C4:

n-Butanol

C8:

n-Octanol

C10:

n-Decanol

C12:

n-Dodecanol

SED:

Substrate enzymatic digestibility

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Acknowledgments

The authors would like to acknowledge the financial supports of the National Natural Science Foundation of China (21376100, 21336002), International S&T Cooperation Program of China (2013DFA41670), the National Basic Research Program of China 973 (2012CB215302) and the Fundamental Research Funds for the Central Universities (2014ZG0022). We acknowledge Liu Hao (State Key Laboratory of Pulp and Paper Engineering, SCUT) for giving the CTec2 sample and Lan Tianqing for assistance in analysis.

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Authors and Affiliations

  1. School of Chemistry and Chemical Engineering, South China University of Technology, Guangzhou, China

    Hongming Lou, Huanran Lai, Shun Wu, Xiuli Li, Dongjie Yang, Xueqing Qiu, Jinhao Huang & Conghua Yi

  2. State Key Laboratory of Pulp and Paper Engineering, South China University of Technology, Guangzhou, China

    Hongming Lou, Dongjie Yang & Xueqing Qiu

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  1. Hongming Lou
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  2. Huanran Lai
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  3. Shun Wu
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  4. Xiuli Li
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  5. Dongjie Yang
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  6. Xueqing Qiu
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  7. Jinhao Huang
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  8. Conghua Yi
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Correspondence to Xueqing Qiu.

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Lou, H., Lai, H., Wu, S. et al. Enhancing enzymatic hydrolysis of crystalline cellulose and lignocellulose by adding long-chain fatty alcohols. Cellulose 21, 3361–3369 (2014). https://doi.org/10.1007/s10570-014-0331-2

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