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Cellulose

, Volume 20, Issue 4, pp 1937–1946 | Cite as

Xylanase supplementation on enzymatic saccharification of dilute acid pretreated poplars at different severities

  • Chao Zhang
  • Xinshu Zhuang
  • Zhao Jiang Wang
  • Fred Matt
  • Franz St. John
  • J. Y. ZhuEmail author
Original Paper

Abstract

Three pairs of solid substrates from dilute acid pretreatment of two poplar wood samples were enzymatically hydrolyzed by cellulase preparations supplemented with xylanase. Supplementation of xylanase improved cellulose saccharification perhaps due to improved cellulose accessibility by xylan hydrolysis. Total xylan removal directly affected enzymatic cellulose saccharification. Furthermore, xylan removal by pretreatment and xylanase are indifferent to enzymatic cellulose saccharification. However, more enzymatic xylose and glucose yields were obtained for a substrate with lower xylan content after a severer pretreatment at the same xylanase dosage. The effectiveness of xylanase at increased dosages depended on the substrates structure or accessibility. High xylanase dosages were more effective on well pretreated substrates than on under-pretreated substrates with high xylan content. The application sequence of xylanase and cellulase affected cellulose saccharification. This effect varied with substrate accessibility, perhaps due to competition between xylanase and cellulase binding to the substrate.

Keywords

Xylanase supplementation Enzymatic hydrolysis/saccharification Poplar/hard wood Pretreatment severity Substrate accessibility 

Notes

Acknowledgments

This work was sponsored by the U.S. Forest Service (USFS) through the Program of Woody Biomass, Bioenergy, and Bioproducts (WBBB, 2011), a USDA Small Business Innovative Research (SBIR) Phase II project (Contract Number: 2010-33610-21589) to BioPulping International, and the Agriculture and Food Research Initiative Competitive Grant No. 2011-68005-30416 from the USDA National Institute of Food and Agriculture (NIFA) through the Northwest Advanced Renewables Alliance (NARA). These projects along with the Chinese Scholarship Council (CSC) and the Chinese Academy of Sciences provided financial support to Zhang, Zhuang, and Wang for their visiting appointments at USFS–FPL. We also would like to acknowledge Dr. Ronald Zalesny and his staff at USFS Northern Research Station, Rhinelander, WI, for harvesting the poplar woods.

References

  1. Balakshin M, Capanema E, Gracz H, Chang H, Jameel H (2011) Quantification of lignin-carbohydrate linkages with high-resolution NMR spectroscopy. Planta 233(6):1097–1110CrossRefGoogle Scholar
  2. Bradford M (1976) A rapid and sensitive for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem 72(1–2):248–254CrossRefGoogle Scholar
  3. Chum HL, Johnson DK, Black SK, Overend RP (1990) Pretreatment-catalyst effects of the combined severity parameter. Appl Biochem Biotechnol 24(25):1–14CrossRefGoogle Scholar
  4. Dence CW (1992) The determination of lignin. In: Lin SY, Dence CW (eds) Methods in lignin chemistry. Springer-Verlag, Berlin, pp 33–61CrossRefGoogle Scholar
  5. Fengel D, Wegener G (1984) Wood: chemistry, ultrastructure, reactions. W. de Gruyter, BerlinGoogle Scholar
  6. Goldemberg J (2007) Ethanol for a sustainable energy future. Science 315:808–810CrossRefGoogle Scholar
  7. Harris JF, Saeman JF, Locke EG (1963) Wood as a chemical raw material. In: Browning BL (ed) The chemistry of wood. Interscience Publishers, New York, pp 535–585Google Scholar
  8. Himmel ME, Ding SY, Johnson DK, Adney WS, Nimlos MR, Brady JW, Foust TD (2007) Biomass recalcitrance: engineering plants and enzymes for biofuels production. Science 315(5813):804–807CrossRefGoogle Scholar
  9. Hu J, Arantes V, Saddler JN (2011) The enhancement of enzymatic hydrolysis of lignocellulosic substrates by the addition of accessory enzymes such as xylanase: is it an additive or synergistic effect? Biotechnol Biofuels 4:36CrossRefGoogle Scholar
  10. Humbird D, Davis R, Tao L, Kinchin C, Hsu D, Aden A, Schoen P, Lukas J, Olthof B, Worley M et al (2011) Process design and economics for biochemical conversion of lignocellulosic biomass to ethanol: dilute-acid pretreatment and enzymatic hydrolysis of corn stover. National Renewable Energy Laboratory, Technical Report, NREL/TP-5100-47764, Contract No. DE-AC36-08GO28308, Golden, CO, MayGoogle Scholar
  11. Kuhnel S, Schols HA, Gruppen H (2011) Aiming for the complete utilization of sugar-beet pulp: examination of the effects of mild acid and hydrothermal pretreatment followed by enzymatic digestion. Biotechnol Biofuels 4:14CrossRefGoogle Scholar
  12. Kumar R, Wyman CE (2009a) Effect of xylanase supplementation of cellulase on digestion of corn stover solids prepared by leading pretreatment technologies. Bioresour Technol 100(18):4203–4213CrossRefGoogle Scholar
  13. Kumar R, Wyman CE (2009b) Effects of cellulase and xylanase enzymes on the deconstruction of solids from pretreatment of poplar by leading technologies. Biotechnol Prog 25(2):302–314CrossRefGoogle Scholar
  14. Leu SY, Zhu JY (2013) Substrate-related factors affecting enzymatic saccharification of lignocelluloses: our recent understanding. Bioenergy Res 6. doi: 10.1007/s12155-012-9276-1
  15. Lou H, Zhu JY, Lan TQ, Lai H, Qiu X (2013) pH-induced lignin surface modification to reduce nonspecific cellulase binding and enhance enzymatic saccharification of lignocelluloses. ChemSusChem 6. doi: 10.1002/cssc.201200859
  16. Luo X, Gleisner R, Tian S, Negron J, Horn E, Pan XJ, Zhu JY (2010) Evaluation of mountain beetle infested lodgepole pine for cellulosic ethanol production by SPORL pretreatment. Ind Eng Chem Res 49(17):8258–8266CrossRefGoogle Scholar
  17. Moxley G, Gaspar AR, Higgins D, Xu H (2012) Structural changes of corn stover lignin during acid pretreatment. J Ind Microbiol Biotechnol 39(9):1289–1299CrossRefGoogle Scholar
  18. Murashima K, Kosugi A, Doi RH (2003) Synergistic effects of cellulosomal xylanase and cellulases from clostridium cellulovorans on plant cell wall degradation. J Bacteriol 185(5):1518–1524CrossRefGoogle Scholar
  19. Qing Q, Wyman CE (2011) Supplementation with xylanase and β-xylosidase to reduce xylo-oligomer and xylan inhibition of enzymatic hydrolysis of cellulose and pretreated corn stover. Biotechnol Biofuels 4(1):18Google Scholar
  20. Qing Q, Yang B, Wyman CE (2010) Xylooligomers are strong inhibitors of cellulose hydrolysis by enzymes. Bioresour Technol 101(24):9624–9630CrossRefGoogle Scholar
  21. Scharlemann JPW, Laurance WF (2008) How green are biofuels? Science 319:43–44CrossRefGoogle Scholar
  22. Schell DJ (2012) Progress towards sustainable biofuels: Pilot-scale demonstration of integrated cellulosic ethanol production. Presented at the 2012 AIChE Annual Meeting, Pittsburgh, PA, Oct 28–Nov 2Google Scholar
  23. Selig MJ, Knoshaug EP, Adney WS, Himmel ME, Decker SR (2008) Synergistic enhancement of cellobiohydrolase performance on pretreated corn stover by addition of xylanase and esterase activities. Bioresour Technol 99(11):4997–5005CrossRefGoogle Scholar
  24. Tian S, Zhu W, Gleisner R, Pan XJ, Zhu JY (2011) Comparisons of SPORL and dilute acid pretreatments for sugar and ethanol productions from aspen. Biotechnol Prog 27(2):419–427CrossRefGoogle Scholar
  25. Wang ZJ, Zhu JY, Zalesny RS, Chen KF (2012) Ethanol production from poplar wood through enzymatic saccharification and fermentation by dilute acid and SPORL pretreatments. Fuel 95:606–614CrossRefGoogle Scholar
  26. Wood TM, Bhat M (1988) Methods for measuring cellulase activities. In: Colowick SP, Kaplan NO (eds) Methods in enzymology, vol. 160, biomass (Part a, cellulose and hemicellulose). Academic Press, Inc, New York, pp 87–112CrossRefGoogle Scholar
  27. Yang B, Wyman CE (2004) Effect of xylan and lignin removal by batch and flow through pretreatment on the enzymatic digestibility of corn stover cellulose. Biotechnol Bioeng 86(1):88–95CrossRefGoogle Scholar
  28. Yang B, Wyman CE (2008) Pretreatment: the key to unlocking low-cost cellulosic ethanol. Biofuels Bioprod Biorefin 2:26–40CrossRefGoogle Scholar
  29. Yu P, Mckinon JJ, Maenz DD, Olkowski AA, Racz VJ, Christensen DA (2003) Enzymic release of reducing sugars from oat hulls by cellulase, as influenced by Aspergillus ferulic acid esterase and trichoderma xylanase. J Agric Food Chem 51:218–223CrossRefGoogle Scholar
  30. Zhang DS, Yang Q, Zhu JY, Pan XJ (2013) Sulfite (SPORL) pretreatment of switchgrass for enzymatic saccharification. Bioresour Technol. doi: 10.1016/j.biortech.2012.11.031
  31. Zhao X, Zhang L, Liu D (2012) Biomass recalcitrance. Part I: the chemical compositions and physical structures affecting the enzymatic hydrolysis of lignocellulose. Biofuels Bioprod Biorefin 6(4):465–482CrossRefGoogle Scholar
  32. Zhu JY, Pan XJ (2010) Woody biomass pretreatment for cellulosic ethanol production: technology and energy consumption evaluation. Bioresour Technol 101:4992–5002CrossRefGoogle Scholar
  33. Zhu JY, Zhuang XS (2012) Conceptual net energy output for biofuel production from lignocellulosic biomass through biorefining. Prog Energy Combust Sci 38(4):583–589CrossRefGoogle Scholar
  34. Zhu JY, Wang GS, Pan XJ, Gleisner R (2009) Specific surface to evaluate the efficiencies of milling and pretreatment of wood for enzymatic saccharification. Chem Eng Sci 64(3):474–485CrossRefGoogle Scholar
  35. Zhu JY, Pan XJ, Zalesny RS Jr (2010a) Pretreatment of woody biomass for biofuel production: energy efficiency, technologies and recalcitrance. Appl Microbiol Biotechnol 87:847–857CrossRefGoogle Scholar
  36. Zhu W, Zhu JY, Gleisner R, Pan XJ (2010b) On energy consumption for size-reduction and yield from subsequent enzymatic saccharification of pretreated lodgepole pine. Bioresour Technol 101(8):2782–2792CrossRefGoogle Scholar
  37. Zhu JY, Verrill SP, Liu H, Herian VL, Pan XJ, Rockwood DL (2011) On polydispersity of plant biomass recalcitrance and its effects on pretreatment optimization for sugar production. Bioenergy Res 4(3):201–210CrossRefGoogle Scholar
  38. Zhu W, Houtman CJ, Zhu JY, Gleisner R, Chen KF (2012) Quantitative predictions of bioconversion of aspen by dilute acid and SPORL pretreatments using a unified combined hydrolysis factor (CHF). Process Biochem 47(5):785–791CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht (outside the USA)  2013

Authors and Affiliations

  • Chao Zhang
    • 1
    • 4
  • Xinshu Zhuang
    • 2
    • 4
  • Zhao Jiang Wang
    • 3
    • 4
  • Fred Matt
    • 4
  • Franz St. John
    • 4
  • J. Y. Zhu
    • 4
  1. 1.School of Chemical Engineering and TechnologyTianjin UniversityTianjinChina
  2. 2.Guangzhou Institute of Energy ConversionChinese Academy of SciencesGuangzhouChina
  3. 3.Key Lab of Paper Science and TechnologyShandong Polytechnic UniversityJinanChina
  4. 4.USDA Forest ServiceForest Products LaboratoryMadisonUSA

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