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Determination of xylo-oligosaccharides in enzymatically hydrolysed pulp by liquid chromatography and capillary electrophoresis

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Abstract

Three different commercial β-1,4-endoxylanase preparations were used to hydrolyze bleached kraft pulp. Xylo-oligosaccharides in the produced filtrates were separated and quantified using both high performance liquid chromatography (HPLC) and capillary electrophoresis (CE). All the determinations were performed without sample derivatization. The analytical methods were used to highlight the differences between the enzymes behaviour in terms of hydrolysates, but also to estimate the productivity of xylo-oligosaccharides from kraft pulp when the bleached material would be used in biorefining industry. The research showed that the glycosyl hydrolase family 10 enzyme produced by Aspergillus oryzae released xylobiose and xylotriose from the pulp material. The major oligosaccharides released by the family 11 enzyme produced by Bacillus sp. were xylotriose, xylobiose and xylotetraose. On the contrary, another family 11 enzyme produced by A. oryzae produced also xylose. The HPLC results agreed well with the xylose concentrations obtained after acid hydrolysis. The CE data showed the same trend, but much lower concentrations were identified than with HPLC. At the same time the HPLC method was able to separate only small oligosaccharides, whereas CE could be used for separation of all the xylo-oligosaccharides from xylobiose to xylohexaose. The highest xylo-oligosaccharide yield was achieved with Shearzyme at pH 5 corresponding to 22 % of total xylan from bleached birch kraft pulp.

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Acknowledgments

The research has received funding from the Academy of Finland’s PEGRES-project and the EU FP 7 Project -New products and businesses for the biorefining industry (BIOTULI). Salla Hiltunen MSc and Dr Hannu Alatalo are thanked for performing enzyme activity analyses. Pierre Cassland at Novozymes is thanked for valuable comments about the enzymes and Dr Anthony Bristow is thanked for linguistic revision of the manuscript.

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Authors and Affiliations

  1. Laboratory of Chemistry, Department of Chemical Technology, Lappeenranta University of Technology, P.O. Box 20, 53851, Lappeenranta, Finland

    Sari Metsämuuronen & Heli Sirén

  2. Laboratory of Fiber and Paper Technology, Department of Chemical Technology, Lappeenranta University of Technology, P.O. Box 20, 53851, Lappeenranta, Finland

    Katja Lyytikäinen & Kaj Backfolk

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  1. Sari Metsämuuronen
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  2. Katja Lyytikäinen
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Correspondence to Sari Metsämuuronen.

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Metsämuuronen, S., Lyytikäinen, K., Backfolk, K. et al. Determination of xylo-oligosaccharides in enzymatically hydrolysed pulp by liquid chromatography and capillary electrophoresis. Cellulose 20, 1121–1133 (2013). https://doi.org/10.1007/s10570-013-9886-6

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  • DOI: https://doi.org/10.1007/s10570-013-9886-6

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