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Statistical optimization of culture conditions for enhanced bacterial cellulose production by Gluconoacetobacter hansenii NCIM 2529

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Abstract

In this study we carried out optimization of bacterial cellulose (BC) production from Gluconoacetobacter hansenii NCIM 2529 using a statistical model. Optimization of medium components and physical parameters used for bacterial cellulose production in G. hansenii NCIM 2529 was performed by two-step optimization. Eight culture parameters were screened by the Plackett–Burman design and significant parameters were optimized by the response surface methodology using a central composite design. Through these two-phase experiments, optimization of BC production was carried out for high production. The maximum average productivity of BC with optimized medium was 0.71 g/100 mL upon fermentation in a shake flask. The average BC production was observed to increase by about 44 % using these statistical techniques of media optimization.

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Acknowledgments

The research fellowship to Bhavna Mohite from University Grants Commission, New Delhi under the Research Fellowship in Sciences for meritorious students scheme is greatly acknowledged.

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Authors and Affiliations

  1. School of Life Sciences, North Maharashtra University, P. B. 80, Jalgaon, 425001, Maharashtra, India

    Bhavna V. Mohite & Satish V. Patil

  2. Department of Statistics, North Maharashtra University, P. B. 80, Jalgaon, 425001, Maharashtra, India

    Kirtee K. Kamalja

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  1. Bhavna V. Mohite
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  2. Kirtee K. Kamalja
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  3. Satish V. Patil
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Correspondence to Satish V. Patil.

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Mohite, B.V., Kamalja, K.K. & Patil, S.V. Statistical optimization of culture conditions for enhanced bacterial cellulose production by Gluconoacetobacter hansenii NCIM 2529. Cellulose 19, 1655–1666 (2012). https://doi.org/10.1007/s10570-012-9760-y

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