Cellulose crystals in fibrovascular bundles of sugarcane culms: orientation, size, distortion, and variability
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Cellulose crystals in fibrovascular bundles of sugarcane culms were throughly characterized by X-ray diffraction, with area-detector patterns acquired in fiber geometry. It was observed that microfibril angles are, on average, higher for bundles from pith compared to bundles from intermediate regions and rind, and higher for ratoon canes compared to plant canes. On the other hand, microfibril angles do not differ significantly among sugarcane cultivars, internode positions in the culm, or ratoon cane cut cycles. Broadening analyses of diffraction peaks yield crystal sizes (crystal width from equatorial 200 reflection and apparent crystal lengths from meridional 002 and 004 reflections) similar to other plant species (woods and bamboo). In addition, compared to reference cellulose Iβ, the 200, 110, and 110 diffraction peaks from sugarcane cellulose are notably shifted. These shifts indicate pronounced crystallite distortions, with expanded intersheet spacing d 200 , contracted d 1 1 0 /d 110 , and monoclinic angle γ closer to 90°. Our findings deepen the understanding of the fine structure and variability of sugarcane lignocellulose.
KeywordsCane Microfibril angle Diffraction Cell wall
Authors thank family Grandis from Fazenda Vitória for kind provision of the sugarcane culms. Research supported by LNLS – Brazilian Synchrotron Light Laboratory and LNBio – Brazilian Biosciences National Laboratory (project GAR6293) and by FAPESP (project 2010/05523-3).
- Andersson S, Serimaa R, Paakkari T, Saranpää P, Pesonen E (2003) Crystallinity of wood and the size of cellulose crystallites in Norway spruce (Picea abies). J Wood Sci 49:531–537Google Scholar
- Cortez LAB (ed) (2010) Sugarcane bioethanol—R&D for productivity and sustainability. Blucher, São PauloGoogle Scholar
- Delhez R, de Keijser TH, Langford JI, Louër D, Mittemeijer EJ, Sonneveld EJ (1993) Crystal imperfection broadening and peak shape in the Rietveld method. In: Young RA (ed) The Rietveld method. Oxford University Press, New York, pp 132–166Google Scholar
- Esau K (1977) Anatomy of seed plants. Wiley, New YorkGoogle Scholar
- Ioelovich M (2008) Cellulose as a nanostructured polymer: a short review. BioResources 3:1403–1418Google Scholar
- Kulshreshtha AK, Patil NB, Dweltz NE, Radhakrishnan T (1969) Axial order in ramie. Text Res J 39:1158–1161Google Scholar
- Parameswaran N, Liese W (1976) On the fine structure of bamboo fibres. Wood Sci Tech 10:231–246Google Scholar
- Triana O, Leonard M, Saavedra F, Fernández N, Gálvez G, Peña E (1990) Atlas del bagazo de la caña de azúcar, 1st edn. GEPLACEA, MéxicoGoogle Scholar
- Van Dillewijn C (1951) Botánica de canã de azúcar. Edicion Revolucionaria, Instituto del Libro, La HabanaGoogle Scholar
- Warren BE (1990) X-ray diffraction. Dover Publications, New YorkGoogle Scholar