Fourier transform infrared spectroscopic approach to the study of the secondary cell wall development in cotton fiber
Cotton fiber maturity is a major yield component and an important fiber quality trait that is directly linked to the quantity of cellulose deposited during the secondary cell wall (SCW) biogenesis. Cotton fiber development consists of five major overlapping stages: differentiation, initiation, polar elongation, secondary cell wall development, and maturation. The transition period between 16 and 21 dpa (days post anthesis) is regarded to represent a major developmental stage between the primary cell wall and the SCW. Fourier Transform Infrared spectroscopy was used to investigate the structural changes that occur during the different developmental stages. The IR spectra of fibers harvested at different stages of development (10, 14, 17, 18, 19, 20, 21, 24, 27, 30, 36, 46, and 56 dpa) show the presence of vibrations located at 1,733 cm−1 (C=O stretching originating from esters or amides) and 1,534 cm−1 (NH2 deformation corresponding to proteins or amino acids). The results converge towards the conclusion that the transition phase between the primary cell wall and the secondary cell wall occurs between 17 and 18 dpa in fibers from TX19 cultivar, while this transition occurs between 21 and 24 dpa in fibers from TX55 cultivar.
KeywordsCotton Cell wall Fiber development Cellulose FTIR Maturity
The authors would like to thank the Texas Department of Agriculture/Food and Fibers Research Grant Program.
- Hsieh Y-L, Hu X-P, Nguyen A (1997) Strength and crystalline structure of developing Acala cotton. Textile Res J 67(7):529–536Google Scholar
- McCann MC, Stacey NJ, Wilson R, Roberts K (1993) Orientation of macromolecules in the walls of elongating carrot cells. J Cell Sci 106:1347–1356Google Scholar
- Muller LL, Jacks TJ (1975) Rapid chemical dehydration of samples for electron microscopic examinations. J Histochem Cytochem 23(2):107–110Google Scholar
- Salmén L, Åkerholm M, Hinterstoisser B (2005) Two-dimensional Fourier transform infrared spectroscopy applied to cellulose and paper. In: Dumitriu S (ed) Polysaccharides, structural diversity and functional versatility, 2nd edn. Marcel Dekker, New York, pp 159–187Google Scholar
- Séné CFB, McCann MC, Wilson RH, Grinter R (1994) Fourier-transform Raman and Fourier-transform infrared spectroscopy an investigation of five higher plant cell walls and their components. Plant Physiol 106:1623–1631Google Scholar
- Wilkins TA, Jernstedt JA (1999) Molecular genetics of developing cotton fibers. In: Basra AS (ed) Cotton fibers, developmental biology, quality improvement, and textile processing. Food Products Press, New York, pp 231–269Google Scholar