Cellulose

, Volume 15, Issue 5, pp 703–710 | Cite as

Cellulose–water interactions during enzymatic hydrolysis as studied by time domain NMR

  • Claus Felby
  • Lisbeth G. Thygesen
  • Jan B. Kristensen
  • Henning Jørgensen
  • Thomas Elder
Article

Abstract

The different states and locations of water within the cellulose matrix can be studied by the use of time domain low field NMR. In this work we show how the state and location of water associated with cellulose in filter paper fibers are affected by enzymatic hydrolysis. Three locations of water were identified in the filter paper; (1) bound water associated with the microfibril surfaces and (2) water in the cell wall or cellulose matrix and (3) capillary water in the lumens and between fibers. The different mechanisms of cellulase enzymes can be seen in their effect on the cellulose–water interactions and the synergistic effects between endo- and exo enzymes can be easily detected by time domain NMR. An interesting observation is that it is possible to link the state and location of water within the cellulose fiber with structural changes upon enzymatic hydrolysis.

Keywords

Cellulose Hydrolysis Enzymes Time domain NMR 

References

  1. Araujo CD, MacKay AL, Whittall KP, Hailey JRT (1993) A diffusion model for spin–spin relaxation of compartmentalized water in wood. J Magn Reson B 101:248–261CrossRefGoogle Scholar
  2. Blümich B, Anferova S, Sharma S, Segre AL, Federici C (2003) Degradation of historical paper: nondestructive analysis by the NMRMOUSE. J Magn Reson 161:204–209CrossRefGoogle Scholar
  3. Capitani D, Emanuele MC, Segre AL, Fanelli C, Fabbri AA, Attanasio D (1998) Early detection of enzymatic attack on paper by NMR relaxometry, EPR spectroscopy and X-ray powder spectra. Nord Pulp Paper Res J 13:95–100CrossRefGoogle Scholar
  4. Dourado F, Mota M, Pala H, Gama FM (1999) Effect of cellulase adsorption on the surface and interfacial properties of cellulose. Cellulose 6:265–282CrossRefGoogle Scholar
  5. Elder T, Labbe N, Harper D, Rials T (2006) Time domain-nuclear magnetic resonance study of chars from southern hardwoods. Biomass Bioenergy 30:855–862CrossRefGoogle Scholar
  6. Froix MF, Nelson R (1975) The interaction of water with cellulose from nuclear magnetic resonance relaxation times. Macromolecules 8:726–730CrossRefGoogle Scholar
  7. Hartley ID, Kamke FA, Peemoeller H (1992) Cluster theory for water sorption in wood. Wood sci Technol 26:83–99CrossRefGoogle Scholar
  8. Jorgensen H, Vibe-Pedersen J, Larsen J, Felby C (2007) Liquefaction of lignocellulose at high-solids concentrations. Biotechnol Bioeng 96:862–870CrossRefGoogle Scholar
  9. Kollmann FFP, Côté WA (1968) Principles of wood science and technology. Vol I: Solid wood. Springer-Verlag, BerlinGoogle Scholar
  10. Kristensen JB, Jørgensen H, Felby C (2006) Factors regulating the hydrolysis of biomass at high dry matter levels. In: Twenty eighth symposium on biotechnology for fuels and chemicals, 1–4 May, Nashville, TN NEWGoogle Scholar
  11. Matthews JF, Skopec CE, Mason PE, Zuccato P, Torget RW, Sugiyama J, Himmel ME, Brady JW (2006) Computer simulations of microcrystalline cellulose 1 β. Carbohydr Res 341:138–152CrossRefGoogle Scholar
  12. Menon RS, MacKay AL, Hailey JRT, Bloom M, Burgess AE, Swanson JS (1987) An NMR investigation of the physiological water distribution in wood during drying. J Appl Polym Sci 33:1141–1155CrossRefGoogle Scholar
  13. Provencher SW (1982) Contin: a general purpose constrained regularization program for inverting noisy linear algebraic and integral equations. Comput Phys Commun 27:229–242CrossRefGoogle Scholar
  14. Whittal KP, Mackay AL (1989) Quantitative interpretation of NMR relaxation data. J Magn Reson 84(1):134–152Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Claus Felby
    • 1
  • Lisbeth G. Thygesen
    • 1
  • Jan B. Kristensen
    • 1
  • Henning Jørgensen
    • 1
  • Thomas Elder
    • 2
  1. 1.Faculty of Life Sciences, Center for Forest and LandscapeUniversity of CopenhagenFrederiksberg CDenmark
  2. 2.Alexandria Forestry Center, Southern Research StationUSDA-Forest ServicePinevilleUSA

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