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Man-tailored cellulose-based carriers for invertase immobilization

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Abstract

Cellulose-based carriers Granocel were specially prepared and optimised for covalent immobilization of enzymes. The effects of carrier characteristics such as pore size, chemistry of anchor groups and their density on invertase immobilization efficiency were evaluated. It was found that the preferential adsorption/binding of the enzyme to a carrier during coupling and its activity after immobilization depended on microenvironmental effects created by hydrophilic surface of the carrier, functional groups and their activators. The best preparations (activity approx. 300 U/mL, high storage stability) were obtained for NH2-Granocel activated with glutaraldehyde. It is probably due to Granocel modification with pentaethylenehexamine that gave a 19-atom spacer arm. The enzyme concentration in coupling mixture was optimised as well. The kinetic parameters of sucrose hydrolysis for native and immobilized invertase were evaluated. Compared to the native invertase, K m value of immobilized enzyme was only twice higher with about three times lower substrate inhibition. Reaction runs in a well mixed batch reactors with native and immobilized invertase showed slightly slower reaction rate in the case of the enzyme covalently bound to Granocel. Very good stability of cellulose-based carrier was proved experimentally by 20 successive reaction runs in a batch reactor.

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Acknowledgments

The study was partly supported by Lithuanian State Science and Studies Foundation according to project of “The Program for Development of Industrial Biotechnology in Lithuania, 2007–2010”, Contract No. N-10/2007.

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Authors and Affiliations

  1. Faculty of Chemistry, Wrocław University of Technology, Wybrzeże Wyspiańskiego 27, 50-373, Wrocław, Poland

    Jolanta Bryjak

  2. Faculty of Chemical Technology, Kaunas University of Technology, Radvilenu pl. 19, 50254, Kaunas, Lithuania

    Jolanta Liesiene

  3. Faculty of Chemical and Food Technology, Slovak University of Technology, Radlinskēho 9, 812 37, Bratislava, Slovak Republic

    Vladimír Štefuca

Authors
  1. Jolanta Bryjak
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  2. Jolanta Liesiene
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  3. Vladimír Štefuca
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Correspondence to Jolanta Bryjak.

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Bryjak, J., Liesiene, J. & Štefuca, V. Man-tailored cellulose-based carriers for invertase immobilization. Cellulose 15, 631–640 (2008). https://doi.org/10.1007/s10570-008-9202-z

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  • DOI: https://doi.org/10.1007/s10570-008-9202-z

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