Skip to main content
SpringerLink
Log in

The effects of five types of tea solutions on epiboly process, neural and cardiovascular development, and locomotor capacity of zebrafish

  • Original Article
  • Published:
Cell Biology and Toxicology Aims and scope Submit manuscript

Abstract

The effects of teas on embryonic development are still known little. The objective of this study was to compare and analyze developmental effects of green tea, delicate flavor oolong tea, strong flavor oolong tea, black tea, and pu’er tea using zebrafish embryos. Embryos were exposed in tea solutions from one-cell stage; the morphology, locomotor capacity, and gene expression of embryos or larvae were analyzed. The results showed that either tea could decrease the length of body and the size of head and eyes. The effect of green tea had the most significant effects on morphology. Only green tea disturbed cell movement, epiboly, and nervous system development. All five tea solutions caused heart structure alternations and lowered heart rates, and effects caused by green tea were severe. Green tea inhibited the formation of dorsal aorta and segmental arteries and decreased the velocity and total movement distance of larvae. In conclusion, the toxicity of green tea to epiboly, neural and cardiovascular development, and locomotor capacity is more severe than that of other teas. Our study played a warning role for safety consumption of teas and provided references for further study of tea’s physiological and pharmacological effects and biological activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price includes VAT (France)

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4
Fig. 5
Fig. 6

References

  • Arab L, Khan F, Lam H. Tea consumption and cardiovascular disease risk. Am J Clin Nutr. 2013;98(6 Suppl):1651S–9S.

    Article  CAS  PubMed  Google Scholar 

  • Balentine DA, Wiseman SA, Bouwens LC. The chemistry of tea flavonoids. Crit Rev Food Sci Nutr. 1997;37(8):693–704.

    Article  CAS  PubMed  Google Scholar 

  • Barenys M, Gassmann K, Baksmeier C, Heinz S, Reverte I, Schmuck M, et al. Epigallocatechin gallate (EGCG) inhibits adhesion and migration of neural progenitor cells in vitro. Arch Toxicol. 2016;91(2):1–11.

    Google Scholar 

  • Bedrood Z, Rameshrad M, Hosseinzadeh H. Toxicological effects of Camellia sinensis (green tea): a review. Phytother Res. 2018;32(7):1163–80.

    Article  PubMed  Google Scholar 

  • Benini A, Cignarella F, Calvarini L, Mantovanelli S, Giacopuzzi E, Zizioli D, et al. slc7a6os gene plays a critical role in defined areas of the developing CNS in zebrafish. Plos One. 2015;10(3):e0119696.

    Article  PubMed Central  PubMed  CAS  Google Scholar 

  • Borday C, Chatonnet F, Thoby-Brisson M, Champagnat J, Fortin G. Neural tube patterning by Krox20 and emergence of a respiratory control. Respir Physiol Neurobiol. 2005;149(1–3):63–72.

    Article  CAS  PubMed  Google Scholar 

  • Fujiki H, Suganuma M. Green tea: an effective synergist with anticancer drugs for tertiary cancer prevention. Cancer Lett. 2012;324(2):119–25.

    Article  CAS  PubMed  Google Scholar 

  • Grove KA, Lambert JD. Laboratory, epidemiological, and human intervention studies show that tea (Camellia sinensis) may be useful in the prevention of obesity. J Nutr. 2010;140(3):446–53.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Heber D, Zhang Y, Yang J, Ma JE, Henning SM, Li Z. Green tea, black tea, and oolong tea polyphenols reduce visceral fat and inflammation in mice fed high-fat, high-sucrose obesogenic diets. J Nutr. 2014;144(9):1385–93.

    Article  CAS  PubMed  Google Scholar 

  • Hodgson JM, Croft KD. Tea flavonoids and cardiovascular health. Mol Asp Med. 2010;31(6):495–502.

    Article  CAS  Google Scholar 

  • Jeng KC, Chen CS, Fang YP, Hou RC, Chen YS. Effect of microbial fermentation on content of statin, GABA, and polyphenols in Pu-Erh tea. J Agric Food Chem. 2007;55(21):8787–92.

    Article  CAS  PubMed  Google Scholar 

  • Kalueff AV, Stewart AM, Gerlai R. Zebrafish as an emerging model for studying complex brain disorders. Trends Pharmacol Sci. 2014;35(2):63–75.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Kane DA, Hammerschmidt M, Mullins MC, Maischein HM, Brand M, van Eeden FJ, et al. The zebrafish epiboly mutants. Development. 1996;123:47–55.

    CAS  PubMed  Google Scholar 

  • Karlstrom RO, Tyurina OV, Kawakami A, Nishioka N, Talbot WS, Sasaki H, et al. Genetic analysis of zebrafish gli1 and gli2 reveals divergent requirements for gli genes in vertebrate development. Development. 2003;130(8):1549–64.

    Article  CAS  PubMed  Google Scholar 

  • Kondo T, Ohta T, Igura K, Hara Y, Kaji K. Tea catechins inhibit angiogenesis in vitro, measured by human endothelial cell growth, migration and tube formation, through inhibition of VEGF receptor binding. Cancer Lett. 2002;180(2):139–44.

    Article  CAS  PubMed  Google Scholar 

  • Krauss S, Johansen T, Korzh V, Fjose A. Expression of the zebrafish paired box gene pax [zf-b] during early neurogenesis. Development. 1991;113(4):1193–206.

    CAS  PubMed  Google Scholar 

  • Kris-Etherton PM, Keen CL. Evidence that the antioxidant flavonoids in tea and cocoa are beneficial for cardiovascular health. Curr Opin Lipidol. 2002;13(1):41–9.

    Article  CAS  PubMed  Google Scholar 

  • Li Q, Zhao HF, Zhang ZF, Liu ZG, Pei XR, Wang JB, et al. Long-term green tea catechin administration prevents spatial learning and memory impairment in senescence-accelerated mouse prone-8 mice by decreasing Abeta1-42 oligomers and upregulating synaptic plasticity-related proteins in the hippocampus. Neuroscience. 2009;163(3):741–9.

    Article  CAS  PubMed  Google Scholar 

  • Lin JK, Lin CL, Liang YC, Linshiau S, Juan IM. Survey of catechins, gallic acid, and methylxanthines in green, oolong, pu-erh, and black teas. J Agric Food Chem. 1998;46(9):3635–42.

    Article  CAS  Google Scholar 

  • Lo HM, Hung CF, Huang YY, Wu WB. Tea polyphenols inhibit rat vascular smooth muscle cell adhesion and migration on collagen and laminin via interference with cell-ECM interaction. J Biomed Sci. 2007;14(5):637–45.

    Article  CAS  PubMed  Google Scholar 

  • Marlow F, Gonzalez EM, Yin C, Rojo C, Solnica-Krezel L. No tail co-operates with non-canonical Wnt signaling to regulate posterior body morphogenesis in zebrafish. Development. 2004;131(1):203–16.

    Article  CAS  PubMed  Google Scholar 

  • Namazi Shabestari A, Saeedi Moghaddam S, Sharifi F, Fadayevatan R, Nabavizadeh F, Delavari A, et al. The most prevalent causes of deaths, DALYs, and geriatric syndromes in Iranian elderly people between 1990 and 2010: findings from the global burden of disease study 2010. Arch Iran Med. 2015;18(8):462–79.

    PubMed  Google Scholar 

  • Norton WH. Toward developmental models of psychiatric disorders in zebrafish. Front Neural Circuits. 2013;7:79.

    Article  PubMed Central  PubMed  Google Scholar 

  • Pei DS, Sun YH, Long Y, Zhu ZY. Inhibition of no tail (ntl) gene expression in zebrafish by external guide sequence (EGS) technique. Mol Biol Rep. 2008;35(2):139–43.

    Article  CAS  PubMed  Google Scholar 

  • Pfeffer PL, Gerster T, Lun K, Brand M, Busslinger M. Characterization of three novel members of the zebrafish Pax2/5/8 family: dependency of Pax5 and Pax8 expression on the Pax2.1 (noi) function. Development. 1998;125(16):3063–74.

    CAS  PubMed  Google Scholar 

  • Rahmani AH, Al Shabrmi FM, Allemailem KS, Aly SM, Khan MA. Implications of green tea and its constituents in the prevention of cancer via the modulation of cell signalling pathway. Biomed Res Int. 2015;2015:925640.

    PubMed Central  PubMed  Google Scholar 

  • Rottbauer W, Wessels G, Dahme T, Just S, Trano N, Hassel D, et al. Cardiac myosin light chain-2: a novel essential component of thick-myofilament assembly and contractility of the heart. Circ Res. 2006;99(3):323–31.

    Article  CAS  PubMed  Google Scholar 

  • Sang S, Lambert JD, Ho C-T, Yang CS. The chemistry and biotransformation of tea constituents. Pharmacol Res. 2011;64(2):87–99.

    Article  CAS  PubMed  Google Scholar 

  • Sarma DN, Barrett ML, Chavez ML, Gardiner P, Ko R, Mahady GB, et al. Safety of green tea extracts: a systematic review by the US pharmacopeia. Drug Saf. 2008;31(6):469–84.

    Article  PubMed  Google Scholar 

  • Schmidt M, Schmitz HJ, Baumgart A, Guedon D, Netsch MI, Kreuter MH, et al. Toxicity of green tea extracts and their constituents in rat hepatocytes in primary culture. Food Chem Toxicol. 2005;43(2):307–14.

    Article  CAS  PubMed  Google Scholar 

  • Segner H. Zebrafish (Danio rerio) as a model organism for investigating endocrine disruption. Comp Biochem Physiol C. 2009;149(2):187–95.

    Google Scholar 

  • Shao J, Chen D, Ye Q, Cui J, Li Y, Li L. Tissue regeneration after injury in adult zebrafish: the regenerative potential of the caudal fin. Dev Dyn. 2011;240(5):1271–7.

    Article  CAS  PubMed  Google Scholar 

  • Sharangi AB. Medicinal and therapeutic potentialities of tea (Camellia sinensis L.)—a review. Food Res Int. 2009;42(5–6):529–35.

    Article  CAS  Google Scholar 

  • Stangl V, Lorenz M, Stangl K. The role of tea and tea flavonoids in cardiovascular health. Mol Nutr Food Res. 2006;50(2):218–28.

    Article  CAS  PubMed  Google Scholar 

  • Tang FY, Chiang EP, Shih CJ. Green tea catechin inhibits ephrin-A1-mediated cell migration and angiogenesis of human umbilical vein endothelial cells. J Nutr Biochem. 2007;18(6):391–9.

    Article  CAS  PubMed  Google Scholar 

  • Wang CC, Chu KO, Chong WS, Li WY, Pang CP, Shum AS, et al. Tea epigallocatechin-3-gallate increases 8-isoprostane level and induces caudal regression in developing rat embryos. Free Radic Biol Med. 2007;43(4):519–27.

    Article  CAS  PubMed  Google Scholar 

  • Westerfield M. The zebrafish book: a guide for the laboratory use of zebrafish (Danio rerio): M. Westerfield 2007

  • Wilkinson DG, Bhatt S, Chavrier P, Bravo R, Charnay P. Segment-specific expression of a zinc-finger gene in the developing nervous system of the mouse. Nature. 1989;337(6206):461–4.

    Article  CAS  PubMed  Google Scholar 

  • Yang CS, Wang X, Lu G, Picinich SC. Cancer prevention by tea: animal studies, molecular mechanisms and human relevance. Nat Rev Cancer. 2009;9(6):429–39.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  • Yang CS, Li G, Yang Z, Guan F, Chen A, Ju J. Cancer prevention by tocopherols and tea polyphenols. Cancer Lett. 2013;334(1):79–85.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

Download references

Funding

This research was funded under the Special Fund for Basic Research on Scientific Instruments from the Chinese National Natural Science Foundation (grant no. 61327802) and the Chinese National Natural Science Foundation of China (Grant No. 31770733).

Author information

Author notes

  1. Xu Li, Qiuping Zhang and Xi Peng contributed equally to this work.

Authors and Affiliations

  1. Tianjin Key Laboratory of Tumor Microenvironment and Neurovascular Regulation, Department of Histology and Embryology, School of Medicine, Nankai University, Tianjin, 300071, China

    Xu Li, Qiuping Zhang, Jia Xu, Yuan Zhang & Dongyan Chen

  2. Department of Pathogenic Biology, School of Medicine, Nankai University, Tianjin, 300071, China

    Xi Peng

  3. College of Horticulture, Nanjing Agricultural University, Nanjing, 210095, China

    Jiaojiao Zhu & Yuhua Wang

  4. Beijing Center for Physical and Chemical Analysis, Beijing, 100089, China

    Yunhe An

Authors
  1. Xu Li
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qiuping Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xi Peng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jia Xu
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Yuan Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Jiaojiao Zhu
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. Yuhua Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  8. Yunhe An
    View author publications

    You can also search for this author in PubMed Google Scholar

  9. Dongyan Chen
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding authors

Correspondence to Yuhua Wang, Yunhe An or Dongyan Chen.

Ethics declarations

Conflict of interest

The authors declare that they have no conflict of interest

Additional information

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Electronic supplementary material

ESM 1

(DOCX 1987 kb)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Li, X., Zhang, Q., Peng, X. et al. The effects of five types of tea solutions on epiboly process, neural and cardiovascular development, and locomotor capacity of zebrafish. Cell Biol Toxicol 35, 205–217 (2019). https://doi.org/10.1007/s10565-018-09453-5

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10565-018-09453-5

Keywords

Search

Navigation