Abstract
Benzo-[a]-pyrene (B[a]P) is a family member of polycyclic aromatic hydrocarbons and a widespread environmental pollutant. It is a mammary carcinogen in rodents and contributes to the development of human breast cancer. However, the signal transduction pathways induced by B[a]P and its role in breast cancer progression have not been studied in detail. Here, we demonstrate that B[a]P induces cell migration through a lipoxygenase- and Src-dependent pathway, as well as the activation of focal adhesion kinase, Src, and the extracellular signal-regulated kinase 2 in MDA-MB-231 breast cancer cells. However, B[a]P is not able to promote migration in the mammary nontumorigenic epithelial cells MCF12A. Moreover, B[a]P promotes an increase of αvβ3 integrin–cell surface levels and an increase of metalloproteinase (MMP)-2 and MMP-9 secretions. In summary, our findings demonstrate that B[a]P induces the activation of signal transduction pathways and biological processes involved in the invasion/metastasis process in MDA-MB-231 breast cancer cells.
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Acknowledgments
We are grateful to Nora Ruiz and Pedro Cortes-Reynosa for their technical assistance. This work was supported by a grant from Conacyt (83802) and UC-Mexus Conacyt. R. C-S, S. V-C, and O. G-H are supported by a Conacyt Predoctoral Training Grant.
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Castillo-Sanchez, R., Villegas-Comonfort, S., Galindo-Hernandez, O. et al. Benzo-[a]-pyrene induces FAK activation and cell migration in MDA-MB-231 breast cancer cells. Cell Biol Toxicol 29, 303–319 (2013). https://doi.org/10.1007/s10565-013-9254-1
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DOI: https://doi.org/10.1007/s10565-013-9254-1