Abstract
The protective properties of a prenylated coumarin, umbelliprenin (UMB), on the human lymphocytes DNA lesions were tested. Lymphocytes were isolated from blood samples taken from healthy volunteers. DNA breaks and resistance to H2O2-induced damage were measured using a single-cell microgel electrophoresis technique under alkaline conditions (comet assay). Human lymphocytes were incubated in UMB (10, 25, 50, 100, 200, and 400 μM) alone or a combination of different concentrations of UMB (10, 25, 50, 100, 200, and 400 μM) and 25 μM H2O2. Untreated cells, ascorbic acid (AA; 25, 50, 100, 200, and 400 μM) and H2O2 (25 μM) were considered as negative control, positive control, and the standard antioxidant agent for our study, respectively. Single cells were analyzed with “TriTek Cometscore version 1.5” software. The DNA damage was expressed as percent tail DNA. UMB exhibited a concentration-dependent increase in protection activity against DNA damage induced by 25 μM H2O2 (from 67.28% to 39.17%). The antigenotoxic activity of AA, in the range 0–50 μM, was greater than that of UMB. However, no significant difference (p > 0.05) in the protective activity was found between UMB and AA at concentrations of approximately higher than 50 μM.
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The authors are indebted to the Research Committee of the School of Pharmacy and Research Council of Mashhad University of Medical Sciences, Iran for approval and financial support of this project.
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Soltani, F., Mosaffa, F., Iranshahi, M. et al. Evaluation of antigenotoxicity effects of umbelliprenin on human peripheral lymphocytes exposed to oxidative stress. Cell Biol Toxicol 25, 291–296 (2009). https://doi.org/10.1007/s10565-008-9083-9
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DOI: https://doi.org/10.1007/s10565-008-9083-9