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Micronucleus frequency and lipid peroxidation in Allium sativum root tip cells treated with gibberellic acid and cadmium

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Abstract

Gibberellic acid (GA3) is a very potent hormone whose natural occurrence in plants controls their development. Cadmium is a particularly dangerous pollutant due to its high toxicity and great solubility in water. In this study, the effect of GA3 on Allium sativum root tip cells was investigated in the presence of cadmium. A. sativum root tip cells were exposed to CdNO3 (50, 100, 200 μM), GA3 (10-3 M), both CdNO3 and GA3. Cytogenetic analyses were performed as micronucleus (MN) assay and mitotic index (MI). Lipid peroxidation analysis was also performed in A. sativum root tip cells for determination of membrane damage. MN exhibited a dose-dependent increase in Cd treatments in A. sativum. GA3 significantly reduced the effect of Cd on the MN frequency. MN was observed in GA3 and GA3 + 50 μm Cd treatments at very low frequency. MI slightly decreased in GA3 and GA3 + Cd treatments. MI decreased more in high concentrations of Cd than combined GA3 + Cd treatments. The high concentrations of cadmium induce MN, lipid peroxidation and lead to genotoxicity in A. sativum. Current work reveals that the effect of Cd on genotoxicity can be partially restored with GA3 application.

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Authors and Affiliations

  1. Faculty of Science and Art, Department of Biology, Mersin University, 33343, Mersin, Turkey

    Ayla Çelik, Serpil Ünyayar, Fazilet Özlem Çekiç & Ayşin Güzel

  2. Faculty of Science and Letters, Department of Biology, Mersin University, 33342, Mersin, Turkey

    Ayla Çelik

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  1. Ayla Çelik
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  2. Serpil Ünyayar
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  3. Fazilet Özlem Çekiç
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  4. Ayşin Güzel
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Correspondence to Ayla Çelik.

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Çelik, A., Ünyayar, S., Çekiç, F.Ö. et al. Micronucleus frequency and lipid peroxidation in Allium sativum root tip cells treated with gibberellic acid and cadmium. Cell Biol Toxicol 24, 159–164 (2008). https://doi.org/10.1007/s10565-007-9025-y

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  • DOI: https://doi.org/10.1007/s10565-007-9025-y

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