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Influence of the Procedure to Immobilize Lipase on SBA-15 for Biodiesel Production from Palm Kernel Oil

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Abstract

Microbial lipase from Burkholderia cepacia was immobilized by covalent bond and physical adsorption on SBA-15 mesoporous support and its catalytic efficiency was measured in the formation of fatty acids ethyl esters from palm kernel oil. The immobilized biocatalyst and its support were characterized by X-ray Diffraction, Fourier Transform Infrared Spectroscopy, N2 Adsorption–desorption, and thermal analysis TG/DTG. The immobilization of the enzyme onto the support maintained the typical structure of the SBA-15. From the TG measurements, it can be deduced that about 25% of lipase was immobilized in SBA-15 via covalent bond and 15% was incorporated via physical adsorption. Both immobilized lipase preparations were used to perform the transesterification reactions using different oil: ethanol molar ratios and time. The biocatalyst prepared by physical adsorption promoted better results, measured by the apparent yield (> 90%) and conversion (98.9%) in all reactions, which were also confirmed by viscosity and density data. The regeneration of SBA-15 support was found to be feasible and demonstrated efficiency for 5 times, reducing the associated costs of the process.

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Acknowledgements

Authors are thankful to Conselho Nacional de Desenvolvimento Científico e Tecnológico CNPq (Process Number 448701/2018-6) and to CAPES (Coordenação de Aperfeiçoamento de Pessoal de Nível Superior—Brazil)—Finance Code 001.

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Authors and Affiliations

  1. Universidade do Estado do Rio Grande do Norte, Programa de Pós-Graduação em Ciências Naturais, Mossoró, RN, 59610.210, Brazil

    Francisco G. H. S. Pinto, Francisco R. Fernandes, Vinicius P. S. Caldeira, Luiz Di Souza & Anne G. D. Santos

  2. Escola de Engenharia de Lorena, Universidade de São Paulo, Lorena, SP, 12606810, Brazil

    Heizir F. de Castro

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  1. Francisco G. H. S. Pinto
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  2. Francisco R. Fernandes
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  3. Vinicius P. S. Caldeira
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  4. Heizir F. de Castro
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  5. Luiz Di Souza
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  6. Anne G. D. Santos
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Correspondence to Anne G. D. Santos.

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Luiz Di Souza—In memoriam.

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Pinto, F.G.H.S., Fernandes, F.R., Caldeira, V.P.S. et al. Influence of the Procedure to Immobilize Lipase on SBA-15 for Biodiesel Production from Palm Kernel Oil. Catal Lett 151, 2187–2196 (2021). https://doi.org/10.1007/s10562-020-03510-7

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  • DOI: https://doi.org/10.1007/s10562-020-03510-7

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