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Degradation of Sesame Oil Phenolics Using Magnetic Immobilized Laccase

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Abstract

Phenolic compounds exhibit toxic effects and there are still many challenges to find an efficient way for degradation and removal of phenol especially from food. In the present study, magnetically immobilized laccase was prepared and applied as an efficient heterogeneous biocatalyst for biodegradation of phenol from sesame oil. Laccase was attached covalently to magnetic Fe3O4 nanospheres and nanorods, and the characteristics of the immobilized enzyme were studied. The magnetic supports were analyzed by scanning electron microscopy, X-ray diffraction, vibrating sample magnetometry, and Fourier-transform infrared spectroscopy. Storage stability analysis of immobilized enzyme showed that more than 70% of initial activity was kept after 15 days at 4 °C. More than 70% phenol degradation was achieved and a 60% decrease in the activity of immobilized laccase was observed after 20 independent cycles. The development of enzyme immobilization techniques on magnetic supports may expand the potential applications of heterogeneous biocatalysts in food industries.

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Abbreviations

ABTS:

2,2′-Azino-bis 3-ethylbenzthiazoline-6-sulfonic acid is a peroxidase substrate suitable for enzyme activity measurement.

APTES:

3-Triethoxysilylpropylamine was used for amination

FT-IR:

Fourier-transform infrared

IU:

The enzyme international unit, One U is defined as the amount of the enzyme that transforms one µmole of substrate / minute.

PAMAM:

Poly(amidoamine) is a type of dendrimer containing repetitively branched subunits of amide and amine moieties that is commonly used for surface modification.

PEG:

Polyethylene glycol

TEOS:

Tetraethyl orthosilicate

VSM:

Vibrating sample magnetometer, was used to determine magnetization properties of nanostructured iron oxide supports.

XRD:

X-ray diffraction, was used to study the structure, composition, and physical properties of synthesized MNPs.

SEM:

Scanning electron microscope was used as a sample imaging tool

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Acknowledgements

Support of this study by the research council of University of Guilan is gratefully acknowledged. Also many thanks for so much help to Professor Wolfgang Bensch and his working group at the Institute for Inorganic Chemistry (CAU, Kiel), to PD Dr. Christoph Plieth of Centre of Biochemistry and Molecular Biology (BiMo; CAU, Kiel), and to Professor Mohammad Ali Faramarzi and his working group, especially M. Mogharabi, at Tehran University of Medical Sciences.

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Authors and Affiliations

  1. Department of Chemistry, Faculty of Sciences, University of Guilan, P.O. Box 41335-1914, Rasht, Guilan, Iran

    Reza Amin & Alireza Khorshidi

  2. Institute of Inorganic Chemistry, Kiel University, Max-Eyth Straβe 2, 24118, Kiel, Germany

    Wolfgang Bensch & Svenja Senkale

  3. Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Tehran University of Medical Sciences, P.O. Box 14155–6451, Tehran, 1417614411, Iran

    Mohammad Ali Faramarzi

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  1. Reza Amin
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  2. Alireza Khorshidi
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  3. Wolfgang Bensch
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  4. Svenja Senkale
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Correspondence to Alireza Khorshidi or Mohammad Ali Faramarzi.

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Amin, R., Khorshidi, A., Bensch, W. et al. Degradation of Sesame Oil Phenolics Using Magnetic Immobilized Laccase. Catal Lett 150, 3086–3095 (2020). https://doi.org/10.1007/s10562-020-03226-8

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  • DOI: https://doi.org/10.1007/s10562-020-03226-8

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