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Immobilization of Carbonic Anhydrase on Modified Electrospun Poly(Lactic Acid) Membranes: Quest for Optimum Biocatalytic Performance

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Abstract

Electrospun poly (lactic acid) membranes modified with graphene oxide (GO) and nano sized metal organic frameworks (nMOF) were synthesized, characterized and used for the immobilization of carbonic anhydrase (CA). The mechanical strength and young’s modulus of the membrane were slightly decreased when GO and nMOF were introduced, due to irregularity in the dispersion. On the other hand, the incorporation of GO into the membrane increased its thermal degradation temperature. Enzyme loading was substantially improved with GO and nMOF integration (~1.83 and ~1.57 times higher than that of poly (lactic acid)). Thermal stability, storage stability and reusability of immobilized CA, all of which are important for industrial applications, were also found to be enhanced significantly.

Graphical Abstract

Improved loading, stability and biocatalytic activity of carbonic anhydrase enzyme on modified electrospun membrane are reported

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Acknowledgments

This research was supported financially by the Advanced Biomass R&D Center (ABC) (ABC-2012053875) Grant funded by the Ministry of Education, Science and Technology, Korea.

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Authors and Affiliations

  1. Department of Chemical and Biomolecular Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-Gu, Daejeon, 305-701, Republic of Korea

    Prakash C. Sahoo, Nonni Soraya Sambudi, Seung Bin Park & Jay H. Lee

  2. Department of Civil and Environmental Engineering, Korea Advanced Institute of Science and Technology (KAIST), 291 Daehak-ro, Yuseong-Gu, Daejeon, 305-701, Republic of Korea

    Prakash C. Sahoo & Jong-In Han

Authors
  1. Prakash C. Sahoo
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  2. Nonni Soraya Sambudi
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  3. Seung Bin Park
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  4. Jay H. Lee
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  5. Jong-In Han
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Corresponding authors

Correspondence to Jay H. Lee or Jong-In Han.

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Sahoo, P.C., Sambudi, N.S., Park, S.B. et al. Immobilization of Carbonic Anhydrase on Modified Electrospun Poly(Lactic Acid) Membranes: Quest for Optimum Biocatalytic Performance. Catal Lett 145, 519–526 (2015). https://doi.org/10.1007/s10562-014-1406-2

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  • DOI: https://doi.org/10.1007/s10562-014-1406-2

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