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Improvement of Chloroperoxidase Catalytic Activities by Chitosan and Thioglycolic Acid

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Abstract

The chlorination and oxidation activity of chloroperoxidase (CPO) was enhanced 32 and 20% by chitosan, and oxidation activity 24% by thioglycolic acid, respectively. The pH optimum of chlorination reaction shifted 3 units to a less acidic environment by chitosan (from 2.75 to 5.75). The kinetic parameters indicated both the affinity and specificity of CPO to substrate were improved.

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Acknowledgments

This work is supported by the National Natural Science Foundation of China (20876094).

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Authors and Affiliations

  1. Key Laboratory of Macromolecular Science of Shaanxi Province, Shaanxi Normal University, Xi’an, Shaanxi, People’s Republic of China

    Yucheng Jiang, Mancheng Hu, Shuni Li & Quanguo Zhai

  2. School of Chemistry and Materials Science, Shaanxi Normal University, Chang’an South Road, 199, 710062, Xi’an, Shaanxi, People’s Republic of China

    Chaohong Bai, Yucheng Jiang, Mancheng Hu, Shuni Li & Quanguo Zhai

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  1. Chaohong Bai
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  2. Yucheng Jiang
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  3. Mancheng Hu
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  4. Shuni Li
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  5. Quanguo Zhai
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Correspondence to Yucheng Jiang.

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Bai, C., Jiang, Y., Hu, M. et al. Improvement of Chloroperoxidase Catalytic Activities by Chitosan and Thioglycolic Acid. Catal Lett 129, 457–461 (2009). https://doi.org/10.1007/s10562-008-9823-8

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  • DOI: https://doi.org/10.1007/s10562-008-9823-8

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