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Comparison of human acellular amniotic membranes with acellular amniotic membranes pretreated with MPLA for repair of fascia in rats

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Abstract

A decellularized amniotic membrane matrix is an ideal scaffolding system. This study compared the effectiveness of amniotic membrane and amniotic membrane pretreated with monophosphoryl lipid A (MPLA) for repairing the fascia of the abdomen with tension sutures. A total of 24 rats were randomly divided into three groups of eight each. In the control group, the fascia was repaired only with non-absorbable monofilament yarn under tension. In the second and third groups, in addition to the control treatment, acellular amniotic membrane was used and MPLA pretreated amniotic membrane was used, respectively. At 3, 7 and 14 days after treatment, the fascia repair was examined macroscopically and microscopically in all groups. Macroscopic examination showed that the use of MPLA pretreated significantly different from the other groups only in the fibrin exudate. Changes in the fascia rupture pressure showed significant differences between groups. Group three, which was sutured with MPLA pretreated acellular membrane, showed greater amounts of collagen, monocytes and neovascularization, especially at days 7 and 14. The results show that MPLA pretreated acellular amniotic membrane helped to repair abdominal fascia to some extent.

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Funding

This study was performed with financial support of Shahrekord University of Medical Sciences; Grant No. 5323.

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Author notes

  1. Ramin Baghaee and Shima Rahmati should be considered joint first authors.

Authors and Affiliations

  1. Clinical Research Development Unit, Hajar Hospital, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Ramin Baghaee & Lotfollah Afzali

  2. Cancer Research Center, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Shima Rahmati

  3. Cellular and Molecular Research Center, Basic Health Sciences Institute, Shahrekord University of Medical Sciences, Shahrekord, Iran

    Mehdi Banitalebi

Authors
  1. Ramin Baghaee
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  2. Shima Rahmati
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  3. Mehdi Banitalebi
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  4. Lotfollah Afzali
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All the authors contributed in experiments and data analyzing, and writing the manuscript.

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Correspondence to Lotfollah Afzali.

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The authors declare that there are no conflicts of interest regarding the publication of this paper.

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All experiments were approved by the Ethics and Clinical Studies Research Committee of SKUMS according to Helsinki Declaration (IR.SKUMS.REC.1400.084).

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Baghaee, R., Rahmati, S., Banitalebi, M. et al. Comparison of human acellular amniotic membranes with acellular amniotic membranes pretreated with MPLA for repair of fascia in rats. Cell Tissue Bank 24, 495–501 (2023). https://doi.org/10.1007/s10561-022-10049-x

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  • DOI: https://doi.org/10.1007/s10561-022-10049-x

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