Abstract
In this study, hydroxyapatite (HA) scaffolds were synthesized and characterized, following the osteogenic and angiogenic effects of HA scaffolds with or without endometrial mesenchymal stem stromal cells (hEnSCs) derived Exosomes were investigated in rat animal model with calvaria defect. The X-ray diffraction (XRD) analysis of HA powder formation was confirmed with Joint Corporation of Powder Diffraction Standards (JCPDS) files numbers of 34-0010 and 24-0033A and Ball mill, and sintering manufactured Nano-size particles. Obtained results containing FE-SEM images presented that the surface of scaffolds has a rough and porous structure, which makes them ideal and appropriate for tissue engineering. Additionally, the XRD showed that these scaffolds exhibited a crystallized structure without undergoing phase transformation; meanwhile, manufactured scaffolds consistently release exosomes; moreover, in vivo findings containing hematoxylin–eosin staining, immunohistochemistry, Masson's trichrome staining, and histomorphometric analysis confirmed that our implant has an osteogenic and angiogenic characteristic. So prepared scaffolds containing exosomes can be proposed as a promising substitute in tissue engineering.
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Acknowledgements
This research were supported by Semnan University of medical sciences with grant number of IR.SEMUMS.REC.1399.048.
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Pouya Youseflee: Conceptualization, Methodology; Faezeh esmaeili ranjbar: Investigation, Writing – original draft, data analysis; Marjan Bahraminasab: Writing – original draft; Ali Ghanbari: editing: Davood Rabiei faradonbeh: software and figures; Samaneh Arab: Investigation; Akram Alizadeh: Conceptualization, methodology; Vajihe Taghdiri Nooshabadi: Supervision, Writing – review & editing.
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Youseflee, P., Ranjbar, F.E., Bahraminasab, M. et al. Exosome loaded hydroxyapatite (HA) scaffold promotes bone regeneration in calvarial defect: an in vivo study. Cell Tissue Bank 24, 389–400 (2023). https://doi.org/10.1007/s10561-022-10042-4
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DOI: https://doi.org/10.1007/s10561-022-10042-4