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A novel 3D-electrospun nanofibers-scaffold grafted with Royal Jelly: improve hydrophilicity of the nanofibers-scaffold and proliferation of HUVEC cell line

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Abstract

Tissue engineering is one of the most important medical rehabilitation tools that includes two vital components of scaffolding and cell growth stimulants. Therefore, designing a more intelligent, portable, monitorable, and safe scaffold that can release growth factors is a key step in achieving an acceptable level of cells for treating patients. In this study, a nanofibers-grafted scaffold was prepared with two-nozzle electrospinning to serve as a tissue engineering scaffold. Fundamental physical characterizations were carried out by scanning electron microscopy (SEM), pore diameter determination, and FT-IR. Fundamental physical characterization revealed that the nanofibers-scaffolds grafted with Royal Jelly significantly increased hydrophilicity, but the porosity of the novel-nanofibers did not alter significantly than the nanofibers without Royal Jelly. Based on the MTT assay results, cell growth, survival, and proliferation of the HUVEC Cell line were increased in the nanofibers scaffold grafted with Royal Jelly. Together, these findings highlight the potential of our novel scaffold for tissue engineering applications.

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References

  • Anjum F, Agabalyan NA, Sparks HD, Rosin NL, Kallos MS, Biernaskie J (2017) Biocomposite nanofiber matrices to support ECM remodeling by human dermal progenitors and enhanced wound closure. Sci Rep 7(1):10291

    Article  PubMed  PubMed Central  Google Scholar 

  • Bajgai MP, Aryal S, Bhattarai SR, Bahadur KCR, Kim KW, Kim HY (2008a) Poly (ϵ-caprolactone) grafted dextran biodegradable electrospun matrix: A novel scaffold for tissue engineering. J Appl Polym Sci 108(3):1447–1454

    Article  CAS  Google Scholar 

  • Bajgai MP, Kim K-W, Parajuli DC, Yoo YC, Kim WD, Khil M-S, Kim HY (2008b) In vitro hydrolytic degradation of poly (ɛ-caprolactone) grafted dextran fibers and films. Polym Degrad Stab 93(12):2172–2179

    Article  CAS  Google Scholar 

  • Bogdanov S (2011) Royal Jelly, bee brood: composition, health, medicine: a review. Lipids 3(8):8–19

    Google Scholar 

  • Coombes AGA, Rizzi SC, Williamson M, Barralet JE, Downes S, Wallace WA (2004) Precipitation casting of polycaprolactone for applications in tissue engineering and drug delivery. Biomaterials 25(2):315–325

    Article  CAS  PubMed  Google Scholar 

  • Ding J, Zhang J, Li J, Li D, Xiao C, Xiao H, Yang H, Zhuang X, Chen X (2019) Electrospun polymer biomaterials. Prog Polym Sci 90:1–34

    Article  CAS  Google Scholar 

  • Du X, Fu S, Zhu Y (2018) 3D printing of ceramic-based scaffolds for bone tissue engineering: an overview. J Mater Chem B 6(27):4397–4412

    Article  CAS  PubMed  Google Scholar 

  • Gasic S, Vucevic D, Vasilijic S, Antunovic M, Chinou I, Colic M (2007) Evaluation of the immunomodulatory activities of Royal Jelly components in vitro. Immunopharmacol Immunotoxicol 29(3–4):521–536

    Article  CAS  PubMed  Google Scholar 

  • Gorain B, Tekade M, Kesharwani P, Iyer AK, Kalia K, Tekade RK (2017) The use of nanoscaffolds and dendrimers in tissue engineering. Drug Discov Today 22(4):652–664

    Article  CAS  PubMed  Google Scholar 

  • Jiang H, Fang D, Hsiao BS, Chu B, Chen W (2004) Optimization and characterization of dextran membranes prepared by electrospinning. Biomacromol 5(2):326–333

    Article  CAS  Google Scholar 

  • Jun I, Han H-S, Edwards RJ, Jeon H (2018) Electrospun fibrous scaffolds for tissue engineering: viewpoints on architecture and fabrication. Int J Mol Sci 19(3):745

    Article  PubMed  PubMed Central  Google Scholar 

  • Khazaei M, Ansarian A, Ghanbari E (2018) New findings on biological actions and clinical applications of Royal Jelly: a review. J Diet Suppl 15(5):757–775

    Article  CAS  PubMed  Google Scholar 

  • Koya-Miyata S, Okamoto I, Ushio S, Iwaki K, Ikeda M, Kurimoto M (2004) Identification of a collagen production-promoting factor from an extract of Royal Jelly and its possible mechanism. Biosci Biotechnol Biochem 68(4):767–773

    Article  PubMed  Google Scholar 

  • Lannutti J, Reneker D, Ma T, Tomasko D, Farson D (2007) Electrospinning for tissue engineering scaffolds. Mater Sci Eng C 27(3):504–509

    Article  CAS  Google Scholar 

  • Li X, You R, Zhang Q, Yan S, Luo Z, Qu J, Li M (2021) Engineering vascularized dermal grafts by integrating a biomimetic scaffold and Wharton’s jelly MSC-derived endothelial cells. J Mater Chem B 9(32):6466–6479

    Article  CAS  PubMed  Google Scholar 

  • Liu Y, Zhou G, Liu Z, Guo M, Jiang X, Taskin MB, Zhang Z, Liu J, Tang J, Bai R, Besenbacher F, Chen M, Chen C (2017) Mussel inspired polynorepinephrine functionalized electrospun polycaprolactone microfibers for muscle regeneration. Sci Rep 7(1):8197

    Article  PubMed  PubMed Central  Google Scholar 

  • Madrid APM, Vrech SM, Sanchez MA, Rodriguez AP (2019) Advances in additive manufacturing for bone tissue engineering scaffolds. Mater Sci Eng C 100:631–644

    Article  Google Scholar 

  • Melliou E, Chinou I (2005) Chemistry and bioactivity of Royal Jelly from Greece. J Agric Food Chem 53(23):8987–8992

    Article  CAS  PubMed  Google Scholar 

  • Montanucci P,. di Pasquali C, Ferri I, Pescara T, Pennoni I, Siccu P, Sidoni A, Cervelli V, Basta G, Calafiore R (2017) Human umbilical cord wharton jelly-derived adult mesenchymal stem cells, in biohybrid scaffolds, for experimental skin regeneration. Stem cells international 2017

  • Movahedi M, Asefnejad A, Rafienia M, Khorasani MT (2021) Synthesis of polyurethane/hyaluronic acid/Royal Jelly electrospun scaffold and evaluating its properties for wound healing. J Mazandaran Univ Med Sci 30(192):1–11

    Google Scholar 

  • Mozafari M, Kargozar S, de Santiago GT, Mohammadi MR, Milan PB, Foroutan Koudehi M, Aghabarari B, Nourani MR (2018) Synthesis and characterisation of highly interconnected porous poly (ε-caprolactone)-collagen scaffolds: a therapeutic design to facilitate tendon regeneration. Mater Technol 33(1):29–37

    Article  CAS  Google Scholar 

  • Pan Y, Zhou X, Wei Y, Zhang Q, Wang T, Zhu M, Li W, Huang R, Liu R, Chen J (2017) Small-diameter hybrid vascular grafts composed of polycaprolactone and polydioxanone fibers. Sci Rep 7(1):3615

    Article  PubMed  PubMed Central  Google Scholar 

  • Pant HR, Neupane MP, Pant B, Panthi G, Oh H-J, Lee MH, Kim HY (2011) Fabrication of highly porous poly (ɛ-caprolactone) fibers for novel tissue scaffold via water-bath electrospinning. Colloids Surf B 88(2):587–592

    Article  CAS  Google Scholar 

  • Pham QP, Sharma U, Mikos AG (2006) Electrospinning of polymeric nanofibers for tissue engineering applications: a review. Tissue Eng 12(5):1197–1211

    Article  CAS  PubMed  Google Scholar 

  • Qasim BS, Zafar SM, Najeeb S, Khurshid Z, Shah HA, Husain S, Rehman UI (2018) Electrospinning of Chitosan-Based Solutions for Tissue Engineering and Regenerative Medicine. Int J Mol Sci 19(2):407

    Article  PubMed  PubMed Central  Google Scholar 

  • Rahmani Del Bakhshayesh A, Annabi N, Khalilov R, Akbarzadeh A, Samiei M, Alizadeh E, Alizadeh-Ghodsi M, Davaran S, Montaseri A (2018) Recent advances on biomedical applications of scaffolds in wound healing and dermal tissue engineering. Artif Cells Nanomed Biotechnol 46(4):691–705

    Article  CAS  PubMed  Google Scholar 

  • Ritcharoen W, Thaiying Y, Saejeng Y, Jangchud I, Rangkupan R, Meechaisue C, Supaphol P (2008) Electrospun dextran fibrous membranes. Cellulose 15(3):435–444

    Article  CAS  Google Scholar 

  • Rostami F, Tamjid E, Behmanesh M (2020) Drug-eluting PCL/graphene oxide nanocomposite scaffolds for enhanced osteogenic differentiation of mesenchymal stem cells. Mater Sci Eng C 115:111102

    Article  CAS  Google Scholar 

  • Sabatini AG, Marcazzan GL, Caboni MF, Bogdanov S, Almeida-Muradian LBD (2009) Quality and standardisation of Royal Jelly. J ApiProduct ApiMed Sci 1(1):1–6

    Article  Google Scholar 

  • Sachlos E, Czernuszka JT (2003) Making tissue engineering scaffolds work. Review: the application of solid freeform fabrication technology to the production of tissue engineering scaffolds. Eur Cell Mater 5(29):39–40

    Google Scholar 

  • Schiffman JD, Schauer CL (2008) A review: electrospinning of biopolymer nanofibers and their applications. Polym Rev 48(2):317–352

    Article  CAS  Google Scholar 

  • Shawki MM, Hereba AM, Ghazal A (2010) Formation and characterisation of antimicrobial dextran nanofibers. Romanian J Biophys 20(4):335–346

    CAS  Google Scholar 

  • Tarantilis PA, Pappas CS, Alissandrakis E, Harizanis PC, Polissiou MG (2012) Monitoring of Royal Jelly protein degradation during storage using Fourier-transform infrared (FTIR) spectroscopy. J Apic Res 51(2):185–192

    Article  CAS  Google Scholar 

  • Vural O, Silici S, Aksakal E (2021) The effect of Royal Jelly dietary on growth performance and expression of genes related to growth and immunity of zebrafish, Danio rerio. Aquac Rep 20:100652

    Article  Google Scholar 

  • Wu P, Dai X, Chen K, Li R, Xing Y (2018) Fabrication of regenerated wool keratin/polycaprolactone nanofiber membranes for cell culture. Int J Biol Macromol 114:1168–1173

    Article  CAS  PubMed  Google Scholar 

  • Xu T, Liang Z, Ding B, Feng Q, Fong H (2018) Polymer blend nanofibers containing polycaprolactone as biocompatible and biodegradable binding agent to fabricate electrospun three-dimensional scaffolds/structures. Polymer 151:299–306

    Article  CAS  Google Scholar 

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Acknowledgements

The authors gratefully acknowledge the research council of Kermanshah University of Medical Sciences (Grant No. 980244) for financial support. This work was performed in partial fulfillment of the requirement for Elaheh Adeli sokoot, in the faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.

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Authors and Affiliations

  1. Kermanshah University of Medical Sciences, Kermanshah, Iran

    Elaheh Adeli Sokoot

  2. Nano Drug Delivery Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, 6734667149, Iran

    Elham Arkan & Pouran Moradipour

  3. Fertility and Infertility Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, Iran

    Mozafar Khazaei

  4. Pharmaceutical Sciences Research Center, Health Technology Institute, Kermanshah University of Medical Sciences, Kermanshah, 6734667149, Iran

    Pouran Moradipour

Authors
  1. Elaheh Adeli Sokoot
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  2. Elham Arkan
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  3. Mozafar Khazaei
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  4. Pouran Moradipour
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Contributions

Processed data, evaluated data and written the manuscript: EAS and EA; Performed experimental research and contributed to the study of the results: MKH and EA; Engaged in experimental work and analyzed data: EAS and EA; Engaged in experimental work and updated manuscript: PM and EAS; Selected suitable animal, rated embryos and gathered research data: FA and LB; Planned tests, analyzed data; and revised the manuscript: EA, PM and MKH.

Corresponding authors

Correspondence to Elham Arkan or Mozafar Khazaei.

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The authors have no conflict of interest to disclose.

Ethical approval

All experiments have been licensed by the Ethical Committee of the Kermanshah University of Medical Sciences, Kermanshah, Iran (KUMS.REC.1396.129).

Data availability statement

The datasets analyzed during the current study are available from the corresponding author on reasonable request.

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Sokoot, E.A., Arkan, E., Khazaei, M. et al. A novel 3D-electrospun nanofibers-scaffold grafted with Royal Jelly: improve hydrophilicity of the nanofibers-scaffold and proliferation of HUVEC cell line. Cell Tissue Bank 24, 329–340 (2023). https://doi.org/10.1007/s10561-022-10035-3

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